ArticlePLoS neglected tropical diseases2011
A proteomic and cellular analysis of uropods in the pathogen Entamoeba histolytica.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
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- Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica.PLoS pathogens · 2024Article
- Fibronectin induces a transition from amoeboid to a fan morphology and modifies migration in Entamoeba histolytica.PLoS pathogens · 2024Article
- Live Cells Imaging and Comparative Phosphoproteomics Uncover Proteins from the Mechanobiome inInternational journal of molecular sciences · 2023Article
- EhVps23, an ESCRT-I Member, Is a Key Factor in Secretion, Motility, Phagocytosis and Tissue Invasion byFrontiers in cellular and infection microbiology · 2022Article
- Cdc42/Rac Interactive Binding Containing Effector Proteins in Unicellular Protozoans With Reference to Human Host: Locks of the Rho Signaling.Frontiers in genetics · 2022Review
- Bioimage Analysis and Cell Motility.Patterns (New York, N.Y.) · 2021Review
- Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe.Foods (Basel, Switzerland) · 2020Review
- Heavy subunit of cell surface Gal/GalNAc lectin (Hgl) undergoes degradation via endo-lysosomal compartments inSmall GTPases · 2019Article
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- Influence of Micropatterned Grill Lines onFrontiers in cellular and infection microbiology · 2018Article
- Morphodynamics of the Actin-Rich Cytoskeleton inFrontiers in cellular and infection microbiology · 2018Article
- Endoplasmic reticulum-resident Rab8A GTPase is involved in phagocytosis in the protozoan parasite Entamoeba histolytica.Cellular microbiology · 2016Article
- Entamoeba histolytica and E. dispar Calreticulin: inhibition of classical complement pathway and differences in the level of expression in amoebic liver abscess.BioMed research international · 2014Article
- The cell surface proteome of Entamoeba histolytica.Molecular & cellular proteomics : MCP · 2014Article
- Localization of phosphatidylinositol 4,5-bisphosphate to lipid rafts and uroids in the human protozoan parasite Entamoeba histolytica.Infection and immunity · 2013Article
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- Entamoeba histolytica Rho1 regulates actin polymerization through a divergent, diaphanous-related formin.Biochemistry · 2012Article
- A novel extracellular metallopeptidase domain shared by animal host-associated mutualistic and pathogenic microbes.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exposure of Entamoeba histolytica to specific ligands induces cell polarization via the activation of signalling pathways and cytoskeletal elements. The process leads to formation of a protruding pseudopod at the front of the cell and a retracting uropod at the rear. In the present study, we show that the uropod forms during the exposure of trophozoites to serum isolated from humans suffering of amoebiasis. To investigate uropod assembly, we used LC-MS/MS technology to identify protein components in isolated uropod fractions. The galactose/N-acetylgalactosamine lectin, the immunodominant antigen M17 (which is specifically recognized by serum from amoeba-infected persons) and a few other cells adhesion-related molecules were primarily involved. Actin-rich cytoskeleton components, GTPases from the Rac and Rab families, filamin, α-actinin and a newly identified ezrin-moesin-radixin protein were the main factors found to potentially interact with capped receptors. A set of specific cysteine proteases and a serine protease were enriched in isolated uropod fractions. However, biological assays indicated that cysteine proteases are not involved in uropod formation in E. histolytica, a fact in contrast to the situation in human motile immune cells. The surface proteins identified here are testable biomarkers which may be either recognized by the immune system and/or released into the circulation during amoebiasis.
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