ArticlePloS one2011
The fate of chrysotile-induced multipolar mitosis and aneuploid population in cultured lung cancer cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
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- Do Carbon Nanotubes and Asbestos Fibers Exhibit Common Toxicity Mechanisms?Nanomaterials (Basel, Switzerland) · 2022Review
- Asbestos and Intrahepatic Cholangiocarcinoma.Cells · 2020Review
- Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2019Article
- Article
- The clinical significance of anti-mitotic spindle apparatus antibody (MSA) and anti-centromere antibody (ACA) detected in patients with small cell lung cancer (SCLC).American journal of clinical and experimental immunology · 2017Article
- Cytogenetic evaluation of functionalized single-walled carbon nanotube in mice bone marrow cells.Environmental toxicology · 2016Article
- Multipolar mitosis and aneuploidy after chrysotile treatment: a consequence of abscission failure and cytokinesis regression.Oncotarget · 2016Article
- Genotoxicity of multi-walled carbon nanotubes at occupationally relevant doses.Particle and fibre toxicology · 2014Article
- Promotion of lung adenocarcinoma following inhalation exposure to multi-walled carbon nanotubes.Particle and fibre toxicology · 2014Article
- Independent of ErbB1 gene copy number, EGF stimulates migration but is not associated with cell proliferation in non-small cell lung cancer.Cancer cell international · 2013Article
- Single-walled carbon nanotube-induced mitotic disruption.Mutation research · 2012Article
- Intrinsic and chemically-induced daughter number variations in cancer cell lines.Cell cycle (Georgetown, Tex.)Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chrysotile is one of the six types of asbestos, and it is the only one that can still be commercialized in many countries. Exposure to other types of asbestos has been associated with serious diseases, such as lung carcinomas and pleural mesotheliomas. The association of chrysotile exposure with disease is controversial. However, in vitro studies show the mutagenic potential of chrysotile, which can induce DNA and cell damage. The present work aimed to analyze alterations in lung small cell carcinoma cultures after 48 h of chrysotile exposure, followed by 2, 4 and 8 days of recovery in fiber-free culture medium. Some alterations, such as aneuploid cell formation, increased number of cells in G2/M phase and cells in multipolar mitosis were observed even after 8 days of recovery. The presence of chrysotile fibers in the cell cultures was detected and cell morphology was observed by laser scanning confocal microscopy. After 4 and 8 days of recovery, only a few chrysotile fragments were present in some cells, and the cellular morphology was similar to that of control cells. Cells transfected with the GFP-tagged α-tubulin plasmid were treated with chrysotile for 24 or 48 h and cells in multipolar mitosis were observed by time-lapse microscopy. Fates of these cells were established: retention in metaphase, cell death, progression through M phase generating more than two daughter cells or cell fusion during telophase or cytokinesis. Some of them were related to the formation of aneuploid cells and cells with abnormal number of centrosomes.
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