ArticleImmunology and cell biology2011
Two modes of lytic granule fusion during degranulation by natural killer cells.
Article in Immunology and cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Going with the membrane flow: the impact of polarized secretion on bulk plasma membrane flows.The FEBS journal · 2023Review
- Lytic granule exocytosis at immune synapses: lessons from neuronal synapses.Frontiers in immunology · 2023Review
- Mechanically active integrins target lytic secretion at the immune synapse to facilitate cellular cytotoxicity.Nature communications · 2022Article
- Ultrarapid lytic granule release from CTLs activates CaScience advances · 2022Article
- The Expanding Arsenal of Cytotoxic T Cells.Frontiers in immunology · 2022Review
- Current state of NK cell-mediated immunotherapy in chronic lymphocytic leukemia.Frontiers in oncology · 2022Review
- Dysregulation of Metabolic Pathways in Circulating Natural Killer Cells Isolated from Inflammatory Bowel Disease Patients.Journal of Crohn's & colitis · 2021Article
- Various Stages of Immune Synapse Formation Are Differently Dependent on the Strength of the TCR Stimulus.International journal of molecular sciences · 2020Article
- NK Cell-Based Glioblastoma Immunotherapy.Cancers · 2018Review
- Metabolic reprogramming of natural killer cells in obesity limits antitumor responses.Nature immunology · 2018Article
- A calcium optimum for cytotoxic T lymphocyte and natural killer cell cytotoxicity.The Journal of physiology · 2018Article
- Single Degranulations in NK Cells Can Mediate Target Cell Killing.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses.Scientific reports · 2018Article
- Human NK Cells Develop an Exhaustion Phenotype During Polar Degranulation at theFrontiers in immunology · 2018Article
- Inducible T Cell Kinase Regulates the Acquisition of Cytolytic Capacity and Degranulation in CD8Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- NK cells converge lytic granules to promote cytotoxicity and prevent bystander killing.The Journal of cell biology · 2016Article
- ERK1/2, MEK1/2 and p38 downstream signalling molecules impaired in CD56 dim CD16+ and CD56 bright CD16 dim/- natural killer cells in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis patients.Journal of translational medicine · 2016Article
- Chediak-Higashi syndrome: Lysosomal trafficking regulator domains regulate exocytosis of lytic granules but not cytokine secretion by natural killer cells.The Journal of allergy and clinical immunology · 2016Article
- Transmissible cytotoxicity of multiple myeloma cells by cord blood-derived NK cells is mediated by vesicle trafficking.Cell death and differentiation · 2015Article
- Vesicle Motion during Sustained Exocytosis in Chromaffin Cells: Numerical Model Based on Amperometric Measurements.PloS one · 2015Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Lytic granules in cytotoxic lymphocytes, which include T cells and natural killer (NK) cells, are secretory lysosomes that release their content upon fusion with the plasma membrane (PM), a process known as degranulation. Although vesicle exocytosis has been extensively studied in endocrine and neuronal cells, much less is known about the fusion of lytic granules in cytotoxic lymphocytes. Here, we used total internal reflection fluorescence microscopy to examine lytic granules labeled with fluorescently tagged Fas ligand (FasL) in the NK cell line NKL stimulated with phorbol ester and ionomycin and in primary NK cells activated by physiological receptor-ligand interactions. Two fusion modes were observed: complete fusion, characterized by loss of granule content and rapid diffusion of FasL at the PM; and incomplete fusion, characterized by transient fusion pore opening and retention of FasL at the fusion site. The pH-sensitive green fluorescence protein (pHluorin) fused to the lumenal domain of FasL was used to visualize fusion pore opening with a time resolution of 30 ms. Upon incomplete fusion, pHluorin emission lasted several seconds in the absence of noticeable diffusion. Thus, we conclude that lytic granules in NK cells undergo both complete and incomplete fusion with the PM, and propose that incomplete fusion may promote efficient recycling of lytic granule membrane after the release of cytotoxic effector molecules.
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