ArticleProceedings of the National Academy of Sciences of the United States of America2011
Fatty acid production in genetically modified cyanobacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
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Who cites it
181 citing papers in PubMed, 523 citations in OpenAlex.
- Next-generation biodiesel production: catalytic innovations and scalable microalgal biorefinery systems.RSC advances · 2026Review
- Towards sustainable production of biofuels via milking the cells of Synechococcus elongatus PCC 7942 engineered for free fatty acid excretion.Biotechnology for biofuels and bioproducts · 2026Article
- Overexpression of endogenous galactolipases and an efflux transporter enhances the secretion of extracellular free fatty acids by Synechococcus elongatus PCC 7942.Biotechnology for biofuels and bioproducts · 2026Article
- From pond to platform: howJournal of bacteriology · 2026Review
- Engineering Cyanobacteria for High-Yield Photosynthetic Isoprene Production With Long-Term Phenotypic Stability.Plant biotechnology journal · 2026Article
- Environmental Applications of GM Microorganisms: Tiny Critters Posing Huge Challenges for Risk Assessment and Governance.International journal of molecular sciences · 2025Review
- Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation.Microbial cell factories · 2025Article
- Recent progress in the cyanobacterial products and applications of phycocyanins.World journal of microbiology & biotechnology · 2025Review
- The regulatory impact of serine/threonine-specific protein phosphorylation among cyanobacteria.Frontiers in plant science · 2025Review
- Biotechnological Applications of Cyanobacteria: Synechocystis and Synechococcus Strains.Advances in biochemical engineering/biotechnology · 2025Review
- Triple-Gene Overexpression of the AcrA-AcrB-TolC Transporter System inInternational journal of molecular sciences · 2024Article
- Insufficient Acetyl-CoA Pool Restricts the Phototrophic Production of Organic Acids in Model Cyanobacteria.International journal of molecular sciences · 2024Review
- Engineering of bacteria towards programmed autolysis: why, how, and when?Microbial cell factories · 2024Review
- Application of Cyanobacteria as Chassis Cells in Synthetic Biology.Microorganisms · 2024Review
- Machine learning model of the catalytic efficiency and substrate specificity of acyl-ACP thioesterase variants generated from natural andFrontiers in bioengineering and biotechnology · 2024Article
- Recent developments in the production and utilization of photosynthetic microorganisms for food applications.Heliyon · 2023Review
- Incorporation, fate, and turnover of free fatty acids in cyanobacteria.FEMS microbiology reviews · 2023Review
- Marinobacter: A case study in bioelectrochemical chassis evaluation.Microbial biotechnology · 2023Review
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- Characterization of a cyanobacterial rep protein with broad-host range and its utilization for expression vectors.Frontiers in microbiology · 2023Article
121 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To avoid costly biomass recovery in photosynthetic microbial biofuel production, we genetically modified cyanobacteria to produce and secrete fatty acids. Starting with introducing an acyl-acyl carrier protein thioesterase gene, we made six successive generations of genetic modifications of cyanobacterium Synechocystis sp. PCC6803 wild type (SD100). The fatty acid secretion yield was increased to 197 ± 14 mg/L of culture in one improved strain at a cell density of 1.0 × 10(9) cells/mL by adding codon-optimized thioesterase genes and weakening polar cell wall layers. Although these strains exhibited damaged cell membranes at low cell densities, they grew more rapidly at high cell densities in late exponential and stationary phase and exhibited less cell damage than cells in wild-type cultures. Our results suggest that fatty acid secreting cyanobacteria are a promising technology for renewable biofuel production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.