ArticleProceedings of the National Academy of Sciences of the United States of America2011
Anchored clathrate waters bind antifreeze proteins to ice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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113 citing papers in PubMed, 388 citations in OpenAlex.
- Nuclear localisation of a fish-derived ice-binding protein and related transcriptional changes inBiochemistry and biophysics reports · 2026Article
- Structure-Guided Discovery of a Cold-Responsive Antifreeze-like Protein from AntarcticMicroorganisms · 2026Article
- Secondary-Structure-Dependent Cooperation and Interference Between Peptides of Different Chain Lengths in Antifreeze Activity: Insights from Molecular Dynamics Simulations.Foods (Basel, Switzerland) · 2026Article
- Hyperactive ice-binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans.FEBS open bio · 2026Article
- Antifreeze Protein for Freeze-Thaw Durability Enhancement of Cement Mortar: Effects and Action Analysis.Materials (Basel, Switzerland) · 2026Article
- How to measure the activity of antifreeze proteins?Biophysical reviews · 2025Review
- Article
- A horizontally transferred bacterial gene aids the freezing tolerance of Antarctic bdelloid rotifers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Fully Atomistic Molecular Dynamics Simulation of Ice Nucleation Near an Antifreeze Protein.Journal of the American Chemical Society · 2025Article
- Cooperative Role of Conformation and Ice-Binding Groups in Ice Growth Inhibition of Antifreeze Glycoproteins.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- AlphaFold 2, but not AlphaFold 3, predicts confident but unrealistic β-solenoid structures for repeat proteins.Computational and structural biotechnology journal · 2025Article
- Protein-Decorated Reverse Osmosis Membranes with High Gypsum Scaling Resistance.ACS environmental Au · 2024Article
- Scalable robust photothermal superhydrophobic coatings for efficient anti-icing and de-icing in simulated/real environments.Nature communications · 2024Article
- Weakly Hydrated Solute of Mixed Hydrophobic-Hydrophilic Nature.The journal of physical chemistry. B · 2024Article
- Multiscale Molecular Dynamics Simulations of Ice-Binding Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Ice Isn't the Only Crystal in Town: Structure Determination of Ice-Binding Proteins via X-Ray Crystallography.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Divergent Mechanisms of Ice Growth Inhibition by Antifreeze Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.eLife · 2023Article
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.bioRxiv : the preprint server for biology · 2023Article
- A general overview of the multifactorial adaptation to cold: biochemical mechanisms and strategies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Review
53 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism by which antifreeze proteins (AFPs) irreversibly bind to ice has not yet been resolved. The ice-binding site of an AFP is relatively hydrophobic, but also contains many potential hydrogen bond donors/acceptors. The extent to which hydrogen bonding and the hydrophobic effect contribute to ice binding has been debated for over 30 years. Here we have elucidated the ice-binding mechanism through solving the first crystal structure of an Antarctic bacterial AFP. This 34-kDa domain, the largest AFP structure determined to date, folds as a Ca(2+)-bound parallel beta-helix with an extensive array of ice-like surface waters that are anchored via hydrogen bonds directly to the polypeptide backbone and adjacent side chains. These bound waters make an excellent three-dimensional match to both the primary prism and basal planes of ice and in effect provide an extensive X-ray crystallographic picture of the AFPice interaction. This unobstructed view, free from crystal-packing artefacts, shows the contributions of both the hydrophobic effect and hydrogen bonding during AFP adsorption to ice. We term this mode of binding the "anchored clathrate" mechanism of AFP action.
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