ArticleProceedings of the National Academy of Sciences of the United States of America2011
Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Various nuclear reprogramming systems using egg and oocyte materials.The Journal of reproduction and development · 2019Review
- Human exhaled air can efficiently supportAnimal reproduction · 2018Article
- Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes.The Journal of reproduction and development · 2018Article
- Maternal histone acetyltransferase KAT8 is required for porcine preimplantation embryo development.Oncotarget · 2017Article
- Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules.Biology open · 2017Article
- Combined positive effect of oocyte extracts and brilliant cresyl blue stained recipient cytoplasts on epigenetic reprogramming and gene expression in buffalo nuclear transfer embryos.Cytotechnology · 2017Article
- RE1-silencing Transcription Factor (REST) Is Required for Nuclear Reprogramming by Inhibiting Transforming Growth Factor β Signaling Pathway.The Journal of biological chemistry · 2016Article
- Cell-free extract from porcine induced pluripotent stem cells can affect porcine somatic cell nuclear reprogramming.The Journal of reproduction and development · 2015Article
- Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis.Proteome science · 2015Article
- Expression profile of developmentally important genes between hand-made cloned buffalo embryos produced from reprogramming of donor cell with oocytes extract and selection of recipient cytoplast through brilliant cresyl blue staining and in vitro fertilized embryos.Journal of assisted reproduction and genetics · 2014Article
- Sperm and spermatids contain different proteins and bind distinct egg factors.International journal of molecular sciences · 2014Article
- Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes.Journal of cellular and molecular medicine · 2014Article
- Identification and characterization of an oocyte factor required for porcine nuclear reprogramming.The Journal of biological chemistry · 2014Article
- Transcriptional regulation and nuclear reprogramming: roles of nuclear actin and actin-binding proteins.Cellular and molecular life sciences : CMLS · 2013Review
- Proteomic analysis of early reprogramming events in murine somatic cells incubated with Xenopus laevis oocyte extracts demonstrates network associations with induced pluripotency markers.Cellular reprogramming · 2013Article
- Nuclear reprogramming of sperm and somatic nuclei in eggs and oocytes.Reproductive medicine and biology · 2013Article
- Pro-apoptotic Effect of Pifithrin-α on Preimplantation Porcine In vitro Fertilized Embryo Development.Asian-Australasian journal of animal sciences · 2012Article
- Reprogramming and development in nuclear transfer embryos and in interspecific systems.Current opinion in genetics & development · 2012Review
- Early aberrations in chromatin dynamics in embryos produced under in vitro conditions.Cellular reprogramming · 2012Article
- Xenopus egg extract treatment reduced global DNA methylation of donor cells and enhanced somatic cell nuclear transfer embryo development in pigs.BioResearch open access · 2012Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear reprogramming of differentiated cells can be induced by oocyte factors. Despite numerous attempts, these factors and mechanisms responsible for successful reprogramming remain elusive. Here, we identify one such factor, necessary for the development of nuclear transfer embryos, using porcine oocyte extracts in which some reprogramming events are recapitulated. After incubating somatic nuclei in oocyte extracts from the metaphase II stage, the oocyte proteins that were specifically and abundantly incorporated into the nuclei were identified by mass spectrometry. Among 25 identified proteins, we especially focused on a multifunctional protein, DJ-1. DJ-1 is present at a high concentration in oocytes from the germinal vesicle stage until embryos at the four-cell stage. Inhibition of DJ-1 function compromises the development of nuclear transfer embryos but not that of fertilized embryos. Microarray analysis of nuclear transfer embryos in which DJ-1 function is inhibited shows perturbed expression of P53 pathway components. In addition, embryonic arrest of nuclear transfer embryos injected with anti-DJ-1 antibody is rescued by P53 inhibition. We conclude that DJ-1 is an oocyte factor that is required for development of nuclear transfer embryos. This study presents a means for identifying natural reprogramming factors in mammalian oocytes and a unique insight into the mechanisms underlying reprogramming by nuclear transfer.
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