ArticleInvestigative ophthalmology & visual science2011
Morphology and immunoreactivity of retrogradely double-labeled ganglion cells in the mouse retina.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 53 citations in OpenAlex.
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- Amacrine Cells Forming Gap Junctions With Intrinsically Photosensitive Retinal Ganglion Cells: ipRGC Types, Neuromodulator Contents, and Connexin Isoform.Investigative ophthalmology & visual science · 2021Article
- Ocular Pressure-Volume Relationship and Ganglion Cell Death in Glaucoma.OBM neurobiology · 2021Article
- Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae.The Journal of comparative neurology · 2020Article
- The expression and function of TRPV4 channels in primate retinal ganglion cells and bipolar cells.Cell death & disease · 2019Article
- Optic nerve thinning and neurosensory retinal degeneration in the rTg4510 mouse model of frontotemporal dementia.Acta neuropathologica communications · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo examine the specificity and reliability of a retrograde double-labeling technique that was recently established for identification of retinal ganglion cells (GCs) and to characterize the morphology of displaced (d)GCs (dGs).
methodsA mixture of the gap-junction-impermeable dye Lucifer yellow (LY) and the permeable dye neurobiotin (NB) was applied to the optic nerve stump for retrograde labeling of GCs and the cells coupled with them. A confocal microscope was adopted for morphologic observation.
resultsGCs were identified by LY labeling, and they were all clearly labeled by NB. Cells coupled to GCs contained a weak NB signal but no LY. LY and NB revealed axon bundles, somas and dendrites of GCs. The retrogradely identified GCs numbered approximately 50,000 per retina, and they constituted 44% of the total neurons in the ganglion cell layer (GCL). Somas of retrogradely identified dGs were usually negative for glycine, ChAT (choline acetyltransferase), bNOS (brain-type nitric oxidase), GAD (glutamate decarboxylase), and glial markers, and occasionally, they were weakly GABA-positive. dGs averaged 760 per retina and composed 1.7% of total GCs. Sixteen morphologic subtypes of dGs were encountered, three of which were distinct from known GCs. dGs sent dendrites to either sublaminas of the IPL, mostly sublamina a.
conclusionsThe retrograde labeling is reliable for identification of GCs. dGs participate in ON and OFF light pathways but favor the OFF pathway. ChAT, bNOS, glycine, and GAD remain reliable AC markers in the GCL. GCs may couple to GABAergic ACs, and the gap junctions likely pass NB and GABA.
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