Evidence map›Paper›PMID 21481568›Full record

ArticleEpilepsy research2011

Different effects of high- and low-dose phenobarbital on post-stroke seizure suppression and recovery in immature CD1 mice.

Geoffrey J Markowitz, Shilpa D Kadam, Dani R Smith, Michael V Johnston, Anne M Comi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epilepsy research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 3 pooled it
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 3 syntheses or guidelines pooled it, 50 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Review
  5. Article
  6. Article
  7. Article
  8. Poststroke epilepsy: current perspectives on diagnosis and treatment.Neuropsychiatric disease and treatment · 2019
    Review
  9. Anesthesia in Experimental Stroke Research.Translational stroke research · 2016
    Review
  10. Review
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Geoffrey J MarkowitzDept. of Neurology and Developmental Medicine, Kennedy Krieger Institute, Baltimore, MD 21205, USA.
Shilpa D KadamDept. of Neurology and Developmental Medicine, Kennedy Krieger Institute, Baltimore, MD 21205, USA; Dept. of Neuroscience, Kennedy Krieger Institute, Baltimore, MD 21205, USA; Dept. of Neurology, Johns Hopkins Medicine, Baltimore, MD 21205, USA.
Dani R SmithNeurogenetics and Behavior Center, Dept. of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, MD 21218, USA.
Michael V JohnstonDept. of Neurology and Developmental Medicine, Kennedy Krieger Institute, Baltimore, MD 21205, USA; Dept. of Neuroscience, Kennedy Krieger Institute, Baltimore, MD 21205, USA; Dept. of Neurology, Johns Hopkins Medicine, Baltimore, MD 21205, USA; Dept. of Pediatrics, Johns Hopkins Medicine, Baltimore, MD 21205, USA.
Anne M ComiDept. of Neurology and Developmental Medicine, Kennedy Krieger Institute, Baltimore, MD 21205, USA; Dept. of Neurology, Johns Hopkins Medicine, Baltimore, MD 21205, USA; Dept. of Pediatrics, Johns Hopkins Medicine, Baltimore, MD 21205, USA.
Johns Hopkins University · USKennedy Krieger Institute · US

Funding

NEUROGENETICS &BEHAVIOR CENTER--RODENTS /ANIMAL MODELSP40RR017688 · NCRR · JOHNS HOPKINS UNIVERSITY · PI GALLAGHER, MICHELA · 2003 to 2007
$5.9M
NEUROTRANSMITTER INJURY MECHANISMS IN DEVELOPING BRAINR01NS028208 · NINDS · HUGO W. MOSER RES INST KENNEDY KRIEGER · PI JOHNSTON, MICHAEL VAN DOREN · 1990 to 2011
$3.4M
Anticonvulsants, ischemic seizures and regeneration in the immature brainR01NS061969 · NINDS · HUGO W. MOSER RES INST KENNEDY KRIEGER · PI COMI, ANNE M · 2009 to 2013
$1.5M
Ischemic Injury and Neuroprotection in the Immature BrainK02NS052166 · NINDS · HUGO W. MOSER RES INST KENNEDY KRIEGER · PI COMI, ANNE M · 2006 to 2010
$900k
NCRR NIH HHS 1P040 RR017688NCRR NIH HHS P40 RR017688NINDS NIH HHS K02 NS052166NINDS NIH HHS NS061969-01NINDS NIH HHS NS52166-01A1NINDS NIH HHS R01 NS028208NINDS NIH HHS R01 NS061969
6 · The paper itself

Abstract

Neonatal stroke presents with seizures that are usually treated with phenobarbital. We hypothesized that anticonvulsants would attenuate ischemic injury, but that the dose-dependent effects of standard anticonvulsants would impact important age-dependent and injury-dependent consequences. In this study, ischemia induced by unilateral carotid ligation in postnatal day 12 (P12) CD1 mice was immediately followed by an i.p. dose of vehicle, low-dose or high-dose phenobarbital. Severity of acute behavioral seizures was scored. 5-Bromo-2'-deoxyuridine (BrdU) was administered from P18 to P20, behavioral testing performed, and mice perfused at P40. Atrophy quantification and counts of BrdU/NeuN-labeled cells in the dentate gyrus were performed. Blood phenobarbital concentrations were measured. 30mg/kg phenobarbital reduced acute seizures and chronic brain injury, and restored normal weight gain and exploratory behavior. By comparison, 60mg/kg was a less efficacious anticonvulsant, was not neuroprotective, did not restore normal weight gain, and impaired behavioral and cognitive recovery. Hippocampal neurogenesis was not different between treatment groups. These results suggest a protective effect of lower-dose phenobarbital, but a lack of this effect at higher concentrations after stroke in P12 mice.

Indexed as

Age FactorsAnimalsAnimals, NewbornAnticonvulsantsAtrophyBody WeightCognition DisordersDisease Models, AnimalDose-Response Relationship, DrugExploratory BehaviorFemaleFunctional LateralityMaleMental DisordersMiceNeurogenesisAnticonvulsantsPhenobarbital

Identifiers

PMID21481568
PMCPMC3288256
OpenAlexW1967279310

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.