ArticleTransgenic research2012
Functional stacking of three resistance genes against Phytophthora infestans in potato.
Article in Transgenic research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 247 citations in OpenAlex.
- A broad-host-range Rhizobium rhizogenes strain enables transient expression across diverse crop species.PLoS biology · 2026Article
- A built-in brake for plant immunity: Resistance (R) protein-mediated immunity is fine-tuned byScience advances · 2026Article
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- No Cost of Resistance in Wheat Gene Stack Lines Containing 10 Stem Rust Resistance Transgenes.Plant biotechnology journal · 2026Article
- Oomycete plant pathogens: biology, pathogenesis and emerging control strategies.Nature reviews. Microbiology · 2026Review
- Marker-Assisted Selection for Disease Resistance in Potato Breeding in the Ural Region of Russia (2018-2025): Comprehensive Genotyping and Validation of Key Resistance Markers.International journal of molecular sciences · 2026Article
- Deciphering the Molecular Interplay Between RXLR-EncodedInternational journal of molecular sciences · 2025Review
- The receptor-like protein RXEG1 confers broad-spectrum resistance in soybean and cotton by recognizing microbial glycoside hydrolase 12 proteins.Plant biotechnology journal · 2025Article
- Arabidopsis CNL receptor SUT1 confers immunity in hydathodes against the vascular pathogen Xanthomonas campestris pv. campestris.PLoS pathogens · 2025Article
- Marker assisted introgression of bacterial leaf blight and cowpea mosaic virus resistance into an elite cowpea cultivar C-152.Molecular breeding : new strategies in plant improvement · 2025Article
- Plant pattern recognition receptors: from evolutionary insight to engineering.Nature reviews. Genetics · 2025Review
- Potato Late Blight Outbreak: A Study on Advanced Classification Models Based on Meteorological Data.Sensors (Basel, Switzerland) · 2024Article
- Resistance Mechanisms of Plant Pathogenic Fungi to Fungicide, Environmental Impacts of Fungicides, and Sustainable Solutions.Plants (Basel, Switzerland) · 2024Review
- Identification of New Isolates of Phytophthora sojae and Selection of Resistant Soybean Genotypes.The plant pathology journal · 2024Article
- DeepFrontiers in plant science · 2024Article
- T-DNA characterization of genetically modified 3-R-gene late blight-resistant potato events with a novel procedure utilizing the Samplix XdropFrontiers in plant science · 2024Article
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- Breeding strategies for late blight resistance in potato crop: recent developments.Molecular biology reports · 2023Review
- Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors.Nature genetics · 2023Article
- The northern corn leaf blight resistance gene Ht1 encodes an nucleotide-binding, leucine-rich repeat immune receptor.Molecular plant pathology · 2023Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional stacking of broad spectrum resistance (R) genes could potentially be an effective strategy for more durable disease resistance, for example, to potato late blight caused by Phytophthora infestans (Pi). For this reason, three broad spectrum potato R genes (Rpi), Rpi-sto1 (Solanum stoloniferum), Rpi-vnt1.1 (S. venturii) and Rpi-blb3 (S. bulbocastanum) were selected, combined into a single binary vector pBINPLUS and transformed into the susceptible cultivar Desiree. Among the 550 kanamycin resistant regenerants, 28 were further investigated by gene specific PCRs. All regenerants were positive for the nptII gene and 23 of them contained the three Rpi genes, referred to as triple Rpi gene transformants. Detached leaf assay and agro-infiltration of avirulence (Avr) genes showed that the 23 triple Rpi gene transformants were resistant to the selected isolates and showed HR with the three Avr effectors indicating functional stacking of all the three Rpi genes. It is concluded that Avr genes, corresponding to the R genes to be stacked, must be available in order to assay for functionality of each stack component. No indications were found for silencing or any other negative effects affecting the function of the inserted Rpi genes. The resistance spectrum of these 23 triple Rpi gene transformants was, as expected, a sum of the spectra from the three individual Rpi genes. This is the first example of a one-step approach for the simultaneous domestication of three natural R genes against a single disease by genetic transformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.