ArticleMolecular imaging and biology2012
Radiolabeling and in vivo imaging of transplanted renal lineages differentiated from human embryonic stem cells in fetal rhesus monkeys.
Article in Molecular imaging and biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Monitoring biological effects of somatic cell genome editing.Nature reviews. Genetics · 2026Review
- Direct Cell Radiolabeling forChemical reviews · 2022Review
- Nonhuman Primates in Translational Research.Annual review of animal biosciences · 2022Review
- Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization.International journal of molecular sciences · 2021Article
- The NIH Somatic Cell Genome Editing program.Nature · 2021Review
- Radiolabeling strategies for radionuclide imaging of stem cells.Stem cell reviews and reports · 2015Article
- Technology advancement for integrative stem cell analyses.Tissue engineering. Part B, Reviews · 2014Review
- Lysophosphatidic acid protects human mesenchymal stromal cells from differentiation-dependent vulnerability to apoptosis.Tissue engineering. Part A · 2014Article
- Characterization of growth, glomerular number, and tubular proteins in the developing rhesus monkey kidney.Anatomical record (Hoboken, N.J. : 2007) · 2013Article
- Radiolabeling human peripheral blood stem cells for positron emission tomography (PET) imaging in young rhesus monkeys.PloS one · 2013Article
- Center for fetal monkey gene transfer for heart, lung, and blood diseases: an NHLBI resource for the gene therapy community.Human gene therapy · 2012Review
- Stem cells as a tool for breast imaging.Journal of oncology · 2012Article
- Molecular imaging of stem cells: tracking survival, biodistribution, tumorigenicity, and immunogenicity.Theranostics · 2012Article
- PET molecular imaging in stem cell therapy for neurological diseases.European journal of nuclear medicine and molecular imaging · 2011Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
purposeThe goals of this study were to optimize radiolabeling of renal lineages differentiated from human embryonic stem (hES) cells and use noninvasive imaging (positron emission tomography (PET) and bioluminescence imaging (BLI)) to detect the cells in fetal monkeys post-transplant. PROCEDURES: hES cells expressing firefly luciferase (5 × 10(6)) were radiolabeled with the optimized concentration of 10 μCi/ml (64)Cu-PTSM then transplanted under ultrasound guidance into early second trimester fetal monkey kidneys. Fetuses were imaged in utero with PET and tissues collected for analysis 3 days post-transplant. Fetal kidneys were imaged ex vivo (PET and BLI) post-tissue harvest, and serial kidney sections were assessed by PCR for human-specific DNA sequences, fluorescent in situ hybridization (FISH) for human-specific centromere probes, and immunohistochemistry (IHC) to assess engrafted cells.
resultsTransplanted cells were readily imaged in vivo and identified at the site of injection; tissue analyses confirmed the imaging findings. Using a semi-quantitative method, one in approximately 650 cells in the kidney was shown to be of human origin by PCR and FISH.
conclusionsThese studies suggest that hES cells differentiated toward renal lineages can be effectively radiolabeled, transplanted into fetal monkey kidneys under ultrasound guidance, monitored with PET post-transplant, and identified by PET, BLI, PCR, FISH, and IHC post-tissue harvest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.