Evidence map›Paper›PMID 21478895›Full record

ReviewAsian journal of andrology2011

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art.

Md Sharoare Hossain, Anders Johannisson, Margareta Wallgren, Szabolcs Nagy, Amanda Pimenta Siqueira, Heriberto Rodriguez-Martinez

Open access · goldAbstract readReview
In one paragraph

Review in Asian journal of andrology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
10.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 180 citations in OpenAlex.

  1. Trial
  2. Arsenic intervened anarchy in cauda epididymis and spermatozoa of adult rats is amended by casein and pea enriched high protein diet.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

Md Sharoare HossainDepartment of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences (SLU), SE-750 07 Uppsala, Sweden.
Anders Johannisson
Margareta Wallgren
Szabolcs Nagy
Amanda Pimenta Siqueira
Heriberto Rodriguez-Martinez
Swedish University of Agricultural Sciences · SELinköping University · SEQuality Research · USUniversity of Pannonia · HU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

Indexed as

Flow CytometryAcrosomeAnimalsCell SurvivalChromatinLipid PeroxidationMaleMitochondriaOxidative StressSemen AnalysisSemen PreservationSex PreselectionSpermatozoaSperm CapacitationChromatin

Identifiers

PMID21478895
PMCPMC3739346
OpenAlexW1975480698

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.