ArticleCell cycle (Georgetown, Tex.)2011
The cell cycle inhibitor p27Kip¹ controls self-renewal and pluripotency of human embryonic stem cells by regulating the cell cycle, Brachyury and Twist.
Article in Cell cycle (Georgetown, Tex.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 46 citations in OpenAlex.
- Roles Played by YY1 in Embryonic, Adult and Cancer Stem Cells.Stem cell reviews and reports · 2021Review
- Skp2 depletion reduces tumor-initiating properties and promotes apoptosis in synovial sarcoma.Translational oncology · 2020Article
- Article
- High Skp2 expression is associated with a mesenchymal phenotype and increased tumorigenic potential of prostate cancer cells.Scientific reports · 2019Article
- Role of p27Oncotarget · 2018Review
- A Distinct DNA Methylation Shift in a Subset of Glioma CpG Island Methylator Phenotypes during Tumor Recurrence.Cell reports · 2018Article
- Cycling to Meet Fate: Connecting Pluripotency to the Cell Cycle.Frontiers in cell and developmental biology · 2018Review
- Engaging Ly-6A/Sca-1 triggers lipid raft-dependent and -independent responses in CD4Immunity, inflammation and disease · 2017Article
- Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation.Scientific reports · 2017Article
- Unique Regulatory Mechanisms for the Human Embryonic Stem Cell Cycle.Journal of cellular physiology · 2017Review
- Nanotopography controls cell cycle changes involved with skeletal stem cell self-renewal and multipotency.Biomaterials · 2017Article
- Article
- Histone modifications and p53 binding poise the p21 promoter for activation in human embryonic stem cells.Scientific reports · 2016Article
- Article
- The non-canonical functions of p27(Kip1) in normal and tumor biology.Cell cycle (Georgetown, Tex.) · 2016Review
- Initiation of stem cell differentiation involves cell cycle-dependent regulation of developmental genes by Cyclin D.Genes & development · 2016Article
- Attenuation of teratoma formation by p27 overexpression in induced pluripotent stem cells.Stem cell research & therapy · 2016Article
- Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.Oncogene · 2015Article
- Form and function: how estrogen and progesterone regulate the mammary epithelial hierarchy.Journal of mammary gland biology and neoplasia · 2015Review
- Increased culture density is linked to decelerated proliferation, prolonged G1 phase, and enhanced propensity for differentiation of self-renewing human pluripotent stem cells.Stem cells and development · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
Abstract
The continued turn over of human embryonic stem cells (hESC) while maintaining an undifferentiated state is dependent on the regulation of the cell cycle. Here we asked the question if a single cell cycle gene could regulate the self-renewal or pluripotency properties of hESC. We identified that the protein expression of the p27(Kip)¹ cell cycle inhibitor is low in hESC cells and increased with differentiation. By adopting a gain and loss of function strategy we forced or reduced its expression in undifferentiating conditions to define its functional role in self-renewal and pluripotency. Using undifferentiation conditions, overexpression of p27(Kip)¹ in hESC lead to a G₁phase arrest with an enlarged and flattened hESC morphology and consequent loss of self-renewal ability. Loss of p27(Kip)¹ caused an elongated/scatter cell-like phenotype involving up-regulation of Brachyury and Twist gene expression. We demonstrate the novel finding that p27(Kip)¹ protein occupies the Twist1 gene promoter and manipulation of p27(Kip)¹ by gain and loss of function is associated with Twist gene expression changes. These results define p27(Kip)¹ expression levels as critical for self-renewal and pluripotency in hESC and suggest a role for p27(Kip)¹ in controlling an epithelial to mesenchymal transition (EMT) in hESC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.