ArticleJournal of bacteriology2011
Promoter recognition and activation by the global response regulator CbrB in Pseudomonas aeruginosa.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Exclusion of catabolic regulation B (cbrB) gene in Pseudomonas aeruginosa attenuates the virulence at different glucose concentrations.BMC microbiology · 2026Article
- Carbon source-driven downregulation of the sRNA AmiL enhancesVirulence · 2025Article
- The Catabolite Repression Control Protein Crc Regulates the Type III Secretion System Through the Adenylate Cyclase CyaB inMicroorganisms · 2025Article
- Article
- Expression regulation of bacterial lipase genes: a review.Frontiers in microbiology · 2025Review
- Spatial heterogeneity in biofilm metabolism elicited by local control of phenazine methylation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Translational regulation by Hfq-Crc assemblies emerges from polymorphic ribonucleoprotein folding.The EMBO journal · 2023Article
- The phosphodiesterase RmcA contributes to the adaptation of Pseudomonas putida to l-arginine.FEMS microbiology letters · 2023Article
- Review
- Role of Two-Component System Networks in Pseudomonas aeruginosa Pathogenesis.Advances in experimental medicine and biology · 2022Article
- Rewiring of Gene Expression inFrontiers in microbiology · 2022Article
- The Regulatory Functions of σInternational journal of molecular sciences · 2021Review
- Specific and Global RNA Regulators inInternational journal of molecular sciences · 2021Review
- Stabilization of Hfq-mediated translational repression by the co-repressor Crc in Pseudomonas aeruginosa.Nucleic acids research · 2021Article
- Article
- Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida.Scientific reports · 2019Article
- The absence of SigX results in impaired carbon metabolism and membrane fluidity in Pseudomonas aeruginosa.Scientific reports · 2018Article
- The CbrB Regulon: Promoter dissection reveals novel insights into the CbrAB expression network in Pseudomonas putida.PloS one · 2018Article
- Harnessing Metabolic Regulation to Increase Hfq-Dependent Antibiotic Susceptibility inFrontiers in microbiology · 2018Article
- The Azoarcus anaerobius 1,3-Dihydroxybenzene (Resorcinol) Anaerobic Degradation Pathway Is Controlled by the Coordinated Activity of Two Enhancer-Binding Proteins.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Pseudomonas aeruginosa, the CbrA/CbrB two-component system is instrumental in the maintenance of the carbon-nitrogen balance and for growth on carbon sources that are energetically less favorable than the preferred dicarboxylate substrates. The CbrA/CbrB system drives the expression of the small RNA CrcZ, which antagonizes the repressing effects of the catabolite repression control protein Crc, an RNA-binding protein. Dicarboxylates appear to cause carbon catabolite repression by inhibiting the activity of the CbrA/CbrB system, resulting in reduced crcZ expression. Here we have identified a conserved palindromic nucleotide sequence that is present in upstream activating sequences (UASs) of promoters under positive control by CbrB and σ(54) RNA polymerase, especially in the UAS of the crcZ promoter. Evidence for recognition of this palindromic sequence by CbrB was obtained in vivo from mutational analysis of the crcZ promoter and in vitro from electrophoretic mobility shift assays using crcZ promoter fragments and purified CbrB protein truncated at the N terminus. Integration host factor (IHF) was required for crcZ expression. CbrB also activated the lipA (lipase) promoter, albeit less effectively, apparently by interacting with a similar but less conserved palindromic sequence in the UAS of lipA. As expected, succinate caused CbrB-dependent catabolite repression of the lipA promoter. Based on these results and previously published data, a consensus CbrB recognition sequence is proposed. This sequence has similarity to the consensus NtrC recognition sequence, which is relevant for nitrogen control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.