ArticleApplied and environmental microbiology2011
Phosphotransferase system-independent glucose utilization in corynebacterium glutamicum by inositol permeases and glucokinases.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 110 citations in OpenAlex.
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- Exploring Levansucrase Operon Regulating Levan-Type Fructooligosaccharides (L-FOSs) Production inJournal of microbiology and biotechnology · 2024Article
- CRISPR-Driven Genome Engineering for Chorismate- and Anthranilate-AccumulatingJournal of microbiology and biotechnology · 2023Article
- Physiological, Biochemical, and Structural Bioinformatic Analysis of the Multiple Inositol Dehydrogenases from Corynebacterium glutamicum.Microbiology spectrum · 2022Article
- Online estimation of changing metabolic capacities in continuous Corynebacterium glutamicum cultivations growing on a complex sugar mixture.Biotechnology and bioengineering · 2022Article
- From Brown Seaweed to a Sustainable Microbial Feedstock for the Production of Riboflavin.Frontiers in bioengineering and biotechnology · 2022Article
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Evolving a New Efficient Mode of Fructose Utilization for Improved Bioproduction inFrontiers in bioengineering and biotechnology · 2021Article
- Recent Advances in Microbial Production ofBiomolecules · 2020Review
- The glucose uptake systems in Corynebacterium glutamicum: a review.World journal of microbiology & biotechnology · 2020Review
- HrrSA orchestrates a systemic response to heme and determines prioritization of terminal cytochrome oxidase expression.Nucleic acids research · 2020Article
- Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production from mixed sugar.Microbial cell factories · 2020Article
- Revisiting the Growth Modulon ofFrontiers in bioengineering and biotechnology · 2020Article
- Impact of COJournal of bacteriology · 2019Article
- Metabolic engineering of glucose uptake systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production.Journal of industrial microbiology & biotechnology · 2019Article
- Characterization of D-Arabitol as Newly Discovered Carbon Source ofFrontiers in microbiology · 2019Article
- Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose.Microbial cell factories · 2018Article
- Holistic bioengineering: rewiring central metabolism for enhanced bioproduction.The Biochemical journal · 2017Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphoenolpyruvate-dependent glucose phosphorylation via the phosphotransferase system (PTS) is the major path of glucose uptake in Corynebacterium glutamicum, but some growth from glucose is retained in the absence of the PTS. The growth defect of a deletion mutant lacking the general PTS component HPr in glucose medium could be overcome by suppressor mutations leading to the high expression of inositol utilization genes or by the addition of inositol to the growth medium if a glucokinase is overproduced simultaneously. PTS-independent glucose uptake was shown to require at least one of the inositol transporters IolT1 and IolT2 as a mutant lacking IolT1, IolT2, and the PTS component HPr could not grow with glucose as the sole carbon source. Efficient glucose utilization in the absence of the PTS necessitated the overexpression of a glucokinase gene in addition to either iolT1 or iolT2. IolT1 and IolT2 are low-affinity glucose permeases with K(s) values of 2.8 and 1.9 mM, respectively. As glucose uptake and phosphorylation via the PTS differs from glucose uptake via IolT1 or IolT2 and phosphorylation via glucokinase by the requirement for phosphoenolpyruvate, the roles of the two pathways for l-lysine production were tested. The l-lysine yield by C. glutamicum DM1729, a rationally engineered l-lysine-producing strain, was lower than that by its PTS-deficient derivate DM1729Δhpr, which, however, showed low production rates. The combined overexpression of iolT1 or iolT2 with ppgK, the gene for PolyP/ATP-dependent glucokinase, in DM1729Δhpr enabled l-lysine production as fast as that by the parent strain DM1729 but with 10 to 20% higher l-lysine yield.
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