ArticleThe Journal of biological chemistry2011
Rb1 gene inactivation expands satellite cell and postnatal myoblast pools.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
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- Ageing Signatures and Disturbed Muscle Regeneration in Muscle Proteome of Inclusion Body Myositis.Journal of cachexia, sarcopenia and muscle · 2025Article
- Regulation of adult stem cell quiescence and its functions in the maintenance of tissue integrity.Nature reviews. Molecular cell biology · 2023Review
- Skeletal Muscle Regeneration in Cardiotoxin-Induced Muscle Injury Models.International journal of molecular sciences · 2022Review
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- Control of satellite cell function in muscle regeneration and its disruption in ageing.Nature reviews. Molecular cell biology · 2022Review
- A Long Journey before Cycling: Regulation of Quiescence Exit in Adult Muscle Satellite Cells.International journal of molecular sciences · 2022Review
- Stem cell-derived exosomes repair ischemic muscle injury by inhibiting the tumor suppressor Rb1-mediated NLRP3 inflammasome pathway.Signal transduction and targeted therapy · 2021Article
- Beyond What Your Retina Can See: Similarities of Retinoblastoma Function between Plants and Animals, from Developmental Processes to Epigenetic Regulation.International journal of molecular sciences · 2020Review
- A requirement of Polo-like kinase 1 in murine embryonic myogenesis and adult muscle regeneration.eLife · 2019Article
- Mechanisms, Hallmarks, and Implications of Stem Cell Quiescence.Stem cell reports · 2019Review
- Quiescence Entry, Maintenance, and Exit in Adult Stem Cells.International journal of molecular sciences · 2019Review
- Article
- Karyopherin Alpha 1 Regulates Satellite Cell Proliferation and Survival by Modulating Nuclear Import.Stem cells (Dayton, Ohio) · 2016Article
- Factors regulating quiescent stem cells: insights from the intestine and other self-renewing tissues.The Journal of physiology · 2016Review
- Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development.Scientific reports · 2016Article
- MiR-206 Attenuates Denervation-Induced Skeletal Muscle Atrophy in Rats Through Regulation of Satellite Cell Differentiation via TGF-β1, Smad3, and HDAC4 Signaling.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- The Spindle Assembly Checkpoint Safeguards Genomic Integrity of Skeletal Muscle Satellite Cells.Stem cell reports · 2015Article
- Intrinsic and extrinsic mechanisms regulating satellite cell function.Development (Cambridge, England) · 2015Review
- The case for primary salivary rhabdomyosarcoma.Frontiers in oncology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Satellite cells are well known as a postnatal skeletal muscle stem cell reservoir that under injury conditions participate in repair. However, mechanisms controlling satellite cell quiescence and activation are the topic of ongoing inquiry by many laboratories. In this study, we investigated whether loss of the cell cycle regulatory factor, pRb, is associated with the re-entry of quiescent satellite cells into replication and subsequent stem cell expansion. By ablation of Rb1 using a Pax7CreER,Rb1 conditional mouse line, satellite cell number was increased 5-fold over 6 months. Furthermore, myoblasts originating from satellite cells lacking Rb1 were also increased 3-fold over 6 months, while terminal differentiation was greatly diminished. Similarly, Pax7CreER,Rb1 mice exhibited muscle fiber hypotrophy in vivo under steady state conditions as well as a delay of muscle regeneration following cardiotoxin-mediated injury. These results suggest that cell cycle re-entry of quiescent satellite cells is accelerated by lack of Rb1, resulting in the expansion of both satellite cells and their progeny in adolescent muscle. Conversely, that sustained Rb1 loss in the satellite cell lineage causes a deficit of muscle fiber formation. However, we also show that pharmacological inhibition of protein phosphatase 1 activity, which will result in pRb inactivation accelerates satellite cell activation and/or expansion in a transient manner. Together, our results raise the possibility that reversible pRb inactivation in satellite cells and inhibition of protein phosphorylation may provide a new therapeutic tool for muscle atrophy by short term expansion of the muscle stem cells and myoblast pool.
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