ArticleJournal of molecular biology2011
Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast α-Factor Receptor.Biomolecules · 2022Review
- Article
- Oligomerization of yeast α-factor receptor detected by fluorescent energy transfer between ligands.Biophysical journal · 2021Article
- A Paradigm for Peptide Hormone-GPCR Analyses.Molecules (Basel, Switzerland) · 2020Review
- The directed evolution of ligand specificity in a GPCR and the unequal contributions of efficacy and affinity.Scientific reports · 2017Article
- Modulating and evaluating receptor promiscuity through directed evolution and modeling.Protein engineering, design & selection : PEDS · 2017Article
- Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.The Journal of biological chemistry · 2016Article
- Evolution of a G protein-coupled receptor response by mutations in regulatory network interactions.Nature communications · 2016Article
- Analysis of random PCR-originated mutants of the yeast Ste2 and Ste3 receptors.MicrobiologyOpen · 2016Article
- Prediction of G Protein-Coupled Receptors with SVM-Prot Features and Random Forest.Scientifica · 2016Article
- A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs.Methods in enzymology · 2015Article
- Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors.Biochimica et biophysica acta · 2014Review
- Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast.Protein engineering, design & selection : PEDS · 2013Article
- Functional and physical interactions among Saccharomyces cerevisiae α-factor receptors.Eukaryotic cell · 2012Article
- Identifying functionally important conformational changes in proteins: activation of the yeast α-factor receptor Ste2p.Journal of molecular biology · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
We describe a rapid method to probe for mutations in cell surface ligand-binding proteins that affect the environment of bound ligand. The method uses fluorescence-activated cell sorting to screen randomly mutated receptors for substitutions that alter the fluorescence emission spectrum of environmentally sensitive fluorescent ligands. When applied to the yeast α-factor receptor Ste2p, a G protein-coupled receptor, the procedure identified 22 substitutions that red shift the emission of a fluorescent agonist, including substitutions at residues previously implicated in ligand binding and at additional sites. A separate set of substitutions, identified in a screen for mutations that alter the emission of a fluorescent α-factor antagonist, occurs at sites that are unlikely to contact the ligand directly. Instead, these mutations alter receptor conformation to increase ligand-binding affinity and provide signaling in response to antagonists of normal receptors. These results suggest that receptor--agonist interactions involve at least two sites, of which only one is specific for the activated conformation of the receptor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.