ReviewCell biology international2011
Out with the old, in with the new? Comparing methods for measuring protein degradation.
Review in Cell biology international, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- Review
- Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy.Scientific reports · 2024Article
- Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate.eLife · 2024Article
- Curcumin stabilizes p53 by interaction with NAD(P)H:quinone oxidoreductase 1 in tumor-derived cell lines.Redox biology · 2020Article
- Oxidized low-density lipoprotein inhibits the degradation of cyclophilin A via the lysosome in vascular smooth muscle cells.American journal of translational research · 2020Article
- A robust and economical pulse-chase protocol to measure the turnover of HaloTag fusion proteins.The Journal of biological chemistry · 2019Article
- Folding and Misfolding of Human Membrane Proteins in Health and Disease: From Single Molecules to Cellular Proteostasis.Chemical reviews · 2019Article
- Dynamics of protein synthesis and degradation through the cell cycle.Cell cycle (Georgetown, Tex.) · 2019Review
- Dual gain and loss of cullin 3 function mediates familial hyperkalemic hypertension.American journal of physiology. Renal physiology · 2018Article
- Longevity in vivo of primary cell wall cellulose synthases.Plant molecular biology · 2018Article
- Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling.Nature protocols · 2017Article
- Incompatibility of chemical protein synthesis inhibitors with accurate measurement of extended protein degradation rates.Pharmacology research & perspectives · 2017Article
- Predictive biophysical modeling and understanding of the dynamics of mRNA translation and its evolution.Nucleic acids research · 2016Article
- Quantitative chemical proteomics profiling of de novo protein synthesis during starvation-mediated autophagy.Autophagy · 2016Article
- Prefoldin Promotes Proteasomal Degradation of Cytosolic Proteins with Missense Mutations by Maintaining Substrate Solubility.PLoS genetics · 2016Article
- Article
- Ubiquitous Autofragmentation of Fluorescent Proteins Creates Abundant Defective Ribosomal Products (DRiPs) for Immunosurveillance.The Journal of biological chemistry · 2015Article
- SGTA regulates the cytosolic quality control of hydrophobic substrates.Journal of cell science · 2014Article
- Profiling human protein degradome delineates cellular responses to proteasomal inhibition and reveals a feedback mechanism in regulating proteasome homeostasis.Cell research · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Protein degradation is a critical factor in controlling cellular protein abundance. Here, we compare classical methods for determining protein degradation rates to a novel GFP (green fluorescent protein) fusion protein based method that assesses the intrinsic stability of cloned cDNA library products by flow cytometry [Yen et al. (2008) Science 322, 918]. While no method is perfect, we conclude that chimeric gene reporter approaches, though powerful, should be applied cautiously, due principally to GFP (or other reporter tag) interference with protein organelle targeting or incorporation into macromolecular assemblies, both of which cause spuriously high degradation rates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.