ArticleAnalytical chemistry2011
Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Application of microfluidic chips in anticancer drug screening.Bosnian journal of basic medical sciences · 2022Review
- Thermosensitive and Conductive Hybrid Polymer for Real-Time Monitoring of Spheroid Growth and Drug Responses.ACS sensors · 2021Article
- Unbiased High-Throughput Drug Combination Pilot Screening Identifies Synergistic Drug Combinations Effective against Patient-Derived and Drug-Resistant Melanoma Cell Lines.SLAS discovery : advancing life sciences R & D · 2021Article
- Breast tumor-on-chip models: From disease modeling to personalized drug screening.Journal of controlled release : official journal of the Controlled Release Society · 2021Review
- Recent advances in microfluidics for drug screening.Biomicrofluidics · 2019Review
- Hollow Micropillar Array Method for High-Capacity Drug Screening on Filter-Grown Epithelial Cells.Analytical chemistry · 2018Article
- Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays.Micromachines · 2018Article
- High-throughput approaches for screening and analysis of cell behaviors.Biomaterials · 2018Review
- Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays.Journal of visualized experiments : JoVE · 2017Article
- Development of a shear stress-free microfluidic gradient generator capable of quantitatively analyzing single-cell morphology.Biomedical microdevices · 2017Article
- Article
- Three-Dimensional Breast Cancer Models Mimic Hallmarks of Size-Induced Tumor Progression.Cancer research · 2016Article
- Production of Uniform 3D Microtumors in Hydrogel Microwell Arrays for Measurement of Viability, Morphology, and Signaling Pathway Activation.Assay and drug development technologies · 2015Article
- A versatile snap chip for high-density sub-nanoliter chip-to-chip reagent transfer.Scientific reports · 2015Article
- Rapid assessment of drug response in cancer cells using microwell array and molecular imaging.Analytical and bioanalytical chemistry · 2014Article
- Sandwich antibody arrays using recombinant antibody-binding protein L.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Development of functional biomaterials with micro- and nanoscale technologies for tissue engineering and drug delivery applications.Journal of tissue engineering and regenerative medicine · 2014Review
- Spot identification and quality control in cell-based microarrays.ACS combinatorial science · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Traditional high-throughput screening (HTS) is carried out in centralized facilities that require extensive robotic liquid and plate handling equipment. This model of HTS is restrictive as such facilities are not accessible to many researchers. We have designed a simple microarray platform for cell-based screening that can be carried out at the benchtop. The device creates a microarray of 2100 individual cell-based assays in a standard microscope slide format. A microarray of chemical-laden hydrogels addresses a matching array of cell-laden microwells thus creating a microarray of sealed microscale cell cultures each with unique conditions. We demonstrate the utility of the device by screening the extent of apoptosis and necrosis in MCF-7 breast cancer cells in response to exposure to a small library of chemical compounds. From a set of screens we produced a rank order of chemicals that preferentially induce apoptosis over necrosis in MCF-7 cells. Treatment with doxorubicin induced high levels of apoptosis in comparison with staurosporine, ethanol, and hydrogen peroxide, whereas treatment with 100 μM ethanol induced minimal apoptosis with high levels of necrosis. We anticipate broad application of the device for various research and discovery applications as it is easy to use, scalable, and can be fabricated and operated with minimal peripheral equipment.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.