Evidence map›Paper›PMID 21475941›Full record

ReviewDie Naturwissenschaften2011

Enumeration of methanogens with a focus on fluorescence in situ hybridization.

Sanjay Kumar, Sumit Singh Dagar, Ashok Kumar Mohanty, Sunil Kumar Sirohi, Monica Puniya, Ramesh C Kuhad, K P S Sangu, Gareth Wyn Griffith, Anil Kumar Puniya

Abstract readReview
PubMed Publisher
In one paragraph

Review in Die Naturwissenschaften, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Molecular-beacon-based tricomponent probe for SNP analysis in folded nucleic acids.Chemistry (Weinheim an der Bergstrasse, Germany) · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Sanjay KumarDairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India.
Sumit Singh Dagar
Ashok Kumar Mohanty
Sunil Kumar Sirohi
Monica Puniya
Ramesh C Kuhad
K P S Sangu
Gareth Wyn Griffith
Anil Kumar Puniya
National Dairy Research Institute · INInstitute of Biological, Environmental and Rural Sciences · GBUniversity of Delhi · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methanogens, the members of domain Archaea are potent contributors in global warming. Being confined to the strict anaerobic environment, their direct cultivation as pure culture is quite difficult. Therefore, a range of culture-independent methods have been developed to investigate their numbers, substrate uptake patterns, and identification in complex microbial communities. Unlike other approaches, fluorescence in situ hybridization (FISH) is not only used for faster quantification and accurate identification but also to reveal the physiological properties and spatiotemporal dynamics of methanogens in their natural environment. Aside from the methodological aspects and application of FISH, this review also focuses on culture-dependent and -independent techniques employed in enumerating methanogens along with associated problems. In addition, the combination of FISH with micro-autoradiography that could also be an important tool in investigating the activities of methanogens is also discussed.

Indexed as

In Situ Hybridization, FluorescenceArchaeaMicrobiological TechniquesOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionRibonuclease HRNA, Ribosomal, 16SRibonuclease HRNA, Ribosomal, 16S

Identifiers

PMID21475941
OpenAlexW1986703215

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.