ArticleJournal of neurotrauma2011
Microfluidic generation of haptotactic gradients through 3D collagen gels for enhanced neurite growth.
Article in Journal of neurotrauma, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Biophysical control of plasticity and patterning in regeneration and cancer.Cellular and molecular life sciences : CMLS · 2023Review
- Functional bioengineered models of the central nervous system.Nature reviews bioengineering · 2023Review
- Mathematical models of neuronal growth.Biomechanics and modeling in mechanobiology · 2022Review
- Review
- Droplet microfluidic devices for organized stem cell differentiation into germ cells: capabilities and challenges.Biophysical reviews · 2021Review
- Embracing Mechanobiology in Next Generation Organ-On-A-Chip Models of Bone Metastasis.Frontiers in medical technology · 2021Review
- Functionally graded biomaterials for use as model systems and replacement tissues.Advanced functional materials · 2020Article
- Hyaluronic acid-based hydrogels with independently tunable mechanical and bioactive signaling features.Biointerphases · 2020Article
- Layer-By-Layer: The Case for 3D Bioprinting Neurons to Create Patient-Specific Epilepsy Models.Materials (Basel, Switzerland) · 2019Review
- Toward Building the Neuromuscular Junction: In Vitro Models To Study Synaptogenesis and Neurodegeneration.ACS omega · 2019Review
- Advances in ex vivo models and lab-on-a-chip devices for neural tissue engineering.Biomaterials · 2019Review
- Stem cells technology: a powerful tool behind new brain treatments.Drug delivery and translational research · 2018Review
- Local extracellular matrix alignment directs cellular protrusion dynamics and migration through Rac1 and FAK.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Substrate-bound protein gradients to study haptotaxis.Frontiers in bioengineering and biotechnology · 2015Review
- Methacrylation induces rapid, temperature-dependent, reversible self-assembly of type-I collagen.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Electrospinning growth factor releasing microspheres into fibrous scaffolds.Journal of visualized experiments : JoVE · 2014Article
- Article
- Hydrogel discs for digital microfluidics.Biomicrofluidics · 2012Article
- Neural cell type-specific responses to glycomimetic functionalized collagen.Biomaterials · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We adapted a microfluidic system used previously to generate durotactic gradients of stiffness in a 3D collagen gel, to produce haptotactic gradients of adhesive ligands through the collagen gel. Oligopeptide sequences that included bioactive peptide sequences from laminin, YIGSR, or IKVAV, were grafted separately onto type I collagen using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Solutions of peptide-grafted collagen and untreated collagen were then used as source and sink input solutions, respectively, in an H-shaped microfluidic network fabricated using traditional soft lithography. One-dimensional gradients of the peptide-grafted collagen solution were generated in the channel that connected the source and sink channels, and these gradients became immobilized upon self-assembly of the collagen into a 3D fibrillar gel. The slope and average concentration of the gradients were adjusted by changing the concentration of the source solutions and by changing the length of the cross-channel. A separate, underlying channel in the microfluidic construct allowed the introduction of a chick embryo dorsal root ganglion into the network. Neurites from these explants grew significantly longer up steep gradients of YIGSR, but shallow gradients of IKVAV in comparison to untreated collagen controls. When these two gradients were presented in combination, the bias in growth acceleration was the largest and most consistent. No differences were observed in the number of neurites choosing to grow up or down the gradients in any condition. These results suggest that the incorporation of distinct gradients of multiple bioactive ligands can improve directional acceleration of regenerating axons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.