ArticleJournal of the American Society for Mass Spectrometry2011
Characterization of AMPylation on threonine, serine, and tyrosine using mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Profiling of Yeast Saccharomyces cerevisiae Mitochondrial AMPylome Reveals a Regulation of OXPHOS Coupling through ATP Synthase Subunit Delta.Journal of proteome research · 2026Article
- The rise of AMPylation: from bacterial beginnings to modern implications in health and disease.Biochemical Society transactions · 2025Review
- A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism.Nature communications · 2025Article
- Mass spectrometric based detection of protein nucleotidylation in the RNA polymerase of SARS-CoV-2.Communications chemistry · 2021Article
- MS-BasedACS infectious diseases · 2020Article
- Novel polyadenylylation-dependent neutralization mechanism of the HEPN/MNT toxin/antitoxin system.Nucleic acids research · 2020Article
- Enzymes Involved in AMPylation and deAMPylation.Chemical reviews · 2018Review
- A Novel Fic (Filamentation Induced by cAMP) Protein from Clostridium difficile Reveals an Inhibitory Motif-independent Adenylylation/AMPylation Mechanism.The Journal of biological chemistry · 2016Article
- Global Profiling of Huntingtin-associated protein E (HYPE)-Mediated AMPylation through a Chemical Proteomic Approach.Molecular & cellular proteomics : MCP · 2016Article
- Analysis of the Structure and Function of FOX-4 Cephamycinase.Antimicrobial agents and chemotherapy · 2016Article
- High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays.Nature protocols · 2015Article
- Chemical reporters for exploring ADP-ribosylation and AMPylation at the host-pathogen interface.Current opinion in chemical biology · 2014Review
- Copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays.Molecular & cellular proteomics : MCP · 2014Article
- Inhibiting AMPylation: a novel screen to identify the first small molecule inhibitors of protein AMPylation.ACS chemical biology · 2014Article
- What pathogens have taught us about posttranslational modifications.Cell host & microbe · 2013Review
- Adenylylation of mycobacterial Glnk (PII) protein is induced by nitrogen limitation.Tuberculosis (Edinburgh, Scotland) · 2013Article
- A chemical reporter for protein AMPylation.Journal of the American Chemical Society · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recent studies have suggested that adenosine 5'-monophosphate (AMP) post-translational modification of proteins could represent a novel molecular signaling pathway. Mass spectrometric fragmentation characteristics of this modification have not previously been described and studied systematically. In this work, we therefore examined the fragmentation pattern of chemically synthesized peptides containing AMPylated Thr, Ser, and Tyr. The formation of characteristic ions and the influence of collision energy (CE) on the detection of characteristic ions and their relative peak intensity are reported. When peptide with AMPylated Ser/Thr underwent collision induced dissociation (CID), peaks at m/z 348.1, 136.1, and 250.1, fragments with AMP group attached, and fragments consistent with neutral loss of 347 Da were major characteristic ions; fragments consistent with neutral loss of 135 Da or 249 Da were weaker and not always detectable. The observations for Tyr AMPylation followed the same general patterns as those for Ser/Thr modification, with the exception that the ions detected for Tyr AMPylation did not include either the peak at m/z 348.1, or fragments with a mass shift of -347 Da. The results described in this paper highlight a series of diagnostic ions, which can be used not only to confidently identify the AMPylation site based on MS and MS/MS data, but also to selectively scan AMPylated peptides in complex protein mixtures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.