ArticleJournal of the American Society for Mass Spectrometry2011
Qualitative metabolome analysis of human cerebrospinal fluid by 13C-/12C-isotope dansylation labeling combined with liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Day-night fluctuations in choroid plexus transcriptomics and cerebrospinal fluid metabolomics.PNAS nexus · 2023Article
- NMR based CSF metabolomics in tuberculous meningitis: correlation with clinical and MRI findings.Metabolic brain disease · 2022Article
- Recurrent Topics in Mass Spectrometry-Based Metabolomics and Lipidomics-Standardization, Coverage, and Throughput.Analytical chemistry · 2021Review
- Chemical Isotope Labeling LC-MS for Metabolomics.Advances in experimental medicine and biology · 2021Article
- Targeting amine- and phenol-containing metabolites in urine by dansylation isotope labeling and liquid chromatography mass spectrometry for evaluation of bladder cancer biomarkers.Journal of food and drug analysis · 2019Article
- Comprehensive urinary metabolomic characterization of a genetically induced mouse model of prostatic inflammation.International journal of mass spectrometry · 2018Article
- Comparative Evaluation of MS-based Metabolomics Software and Its Application to Preclinical Alzheimer's Disease.Scientific reports · 2018Article
- The Impact of GFP Reporter Gene Transduction and Expression on Metabolomics of Placental Mesenchymal Stem Cells Determined by UHPLC-Q/TOF-MS.Stem cells international · 2017Article
- Liquid Chromatography Mass Spectrometry-Based Metabolite Pathway Analyses of Myeloma and Non-Hodgkin's Lymphoma Patients.Cell journal · 2017Article
- Relative quantification of amine-containing metabolites using isobaric N,N-dimethyl leucine (DiLeu) reagents via LC-ESI-MS/MS and CE-ESI-MS/MS.The Analyst · 2015Article
- Multiplexed analysis of cage and cage free chicken egg fatty acids using stable isotope labeling and mass spectrometry.Molecules (Basel, Switzerland) · 2013Article
- Liquid chromatography/mass spectrometry methods for measuring dipeptide abundance in non-small-cell lung cancer.Rapid communications in mass spectrometry : RCM · 2013Article
- Isotopic labeling-assisted metabolomics using LC-MS.Analytical and bioanalytical chemistry · 2013Article
- Development of isotope labeling LC-MS for human salivary metabolomics and application to profiling metabolome changes associated with mild cognitive impairment.Analytical chemistry · 2012Article
- Relative quantification of biomarkers using mixed-isotope labeling coupled with MS.Bioanalysis · 2012Review
- Isotope enhanced approaches in metabolomics.Advances in experimental medicine and biology · 2012Review
- A hypothetical astrocyte-microglia lactate shuttle derived from aMetabolomics : Official journal of the Metabolomic SocietyArticle
- Biomarker Discovery and Translation in Metabolomics.Current MetabolomicsArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolome analysis of human cerebrospinal fluid (CSF) is challenging because of low abundance of metabolites present in a small volume of sample. We describe and apply a sensitive isotope labeling LC-MS technique for qualitative analysis of the CSF metabolome. After a CSF sample is divided into two aliquots, they are labeled by (13)C-dansyl and (12)C-dansyl chloride, respectively. The differentially labeled aliquots are then mixed and subjected to LC-MS using Fourier-transform ion cyclotron resonance mass spectrometry (FTICR MS). Dansylation offers significant improvement in the performance of chromatography separation and detection sensitivity. Moreover, peaks detected in the mass spectra can be readily analyzed for ion pair recognition and database search based on accurate mass and/or retention time information. It is shown that about 14,000 features can be detected in a 25-min LC-FTICR MS run of a dansyl-labeled CSF sample, from which about 500 metabolites can be profiled. Results from four CSF samples are compared to gauge the detectability of metabolites by this method. About 261 metabolites are commonly detected in replicate runs of four samples. In total, 1132 unique metabolite ion pairs are detected and 347 pairs (31%) matched with at least one metabolite in the Human Metabolome Database. We also report a dansylation library of 220 standard compounds and, using this library, about 85 metabolites can be positively identified. Among them, 21 metabolites have never been reported to be associated with CSF. These results illustrate that the dansylation LC-FTICR MS method can be used to analyze the CSF metabolome in a more comprehensive manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.