ArticleJournal of the American Society for Mass Spectrometry2011
MALDI mass spectrometry imaging of neuronal cell cultures.
Article in Journal of the American Society for Mass Spectrometry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements.Current protein & peptide science · 2021Review
- New techniques, applications and perspectives in neuropeptide research.The Journal of experimental biology · 2018Review
- Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions.Molecular & cellular proteomics : MCP · 2018Article
- Single Cell Profiling Using Ionic Liquid Matrix-Enhanced Secondary Ion Mass Spectrometry for Neuronal Cell Type Differentiation.Analytical chemistry · 2017Article
- High Throughput In Situ DDA Analysis of Neuropeptides by Coupling Novel Multiplex Mass Spectrometric Imaging (MSI) with Gas-Phase Fractionation.Journal of the American Society for Mass Spectrometry · 2015Article
- Lutein and Brain Function.Foods (Basel, Switzerland) · 2015Article
- Defining the Neuropeptidome of the Spiny Lobster Panulirus interruptus Brain Using a Multidimensional Mass Spectrometry-Based Platform.Journal of proteome research · 2015Article
- Chemometric analysis of MALDI mass spectrometric images of three-dimensional cell culture systems.Analytical methods : advancing methods and applications · 2015Article
- Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples.Biochimica et biophysica acta · 2015Review
- Advances in Mass Spectrometric Tools for Probing Neuropeptides.Annual review of analytical chemistry (Palo Alto, Calif.) · 2015Review
- Review of methods to probe single cell metabolism and bioenergetics.Metabolic engineering · 2015Review
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Mass spectrometry imaging with high resolution in mass and space.Histochemistry and cell biology · 2013Review
- A vision for better health: mass spectrometry imaging for clinical diagnostics.Clinica chimica acta; international journal of clinical chemistry · 2013Review
- Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research.Chemical reviews · 2013Review
- Correlated imaging--a grand challenge in chemical analysis.The Analyst · 2013Review
- Single-cell lipidomics: characterizing and imaging lipids on the surface of individual Aplysia californica neurons with cluster secondary ion mass spectrometry.Analytical chemistry · 2013Article
- Semitransparent nanostructured films for imaging mass spectrometry and optical microscopy.Analytical chemistry · 2012Article
- Single-cell metabolomics: changes in the metabolome of freshly isolated and cultured neurons.ACS chemical neuroscience · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mass spectrometry imaging (MSI) provides the ability to detect and identify a broad range of analytes and their spatial distributions from a variety of sample types, including tissue sections. Here we describe an approach for probing neuropeptides from sparse cell cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MSI--at single cell spatial resolution-in both MS and tandem MS modes. Cultures of Aplysia californica neurons are grown on an array of glass beads embedded in a stretchable layer of Parafilm M. As the membrane is stretched, the beads/neurons are separated physically and the separated beads/neurons analyzed via MALDI TOF MS. Compared with direct MS imaging of samples, the stretching procedure enhances analyte extraction and incorporation into the MALDI matrix, with negligible analyte spread between separated beads. MALDI tandem MSI using the stretched imaging approach yields localization maps of both parent and fragment ions from Aplysia pedal peptide, thereby confirming peptide identification. This methodology represents a flexible platform for MSI investigation of a variety of cell cultures, including functioning neuronal networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.