ArticleMolecular and cellular biology1990
Cell cycle arrest caused by CLN gene deficiency in Saccharomyces cerevisiae resembles START-I arrest and is independent of the mating-pheromone signalling pathway.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 142 citations in OpenAlex.
- A MAP kinase cascade modulates expression of late G1 phase cyclins in budding yeast.bioRxiv : the preprint server for biology · 2025Article
- Evidence for novel mechanisms that control cell-cycle entry and cell size.Molecular biology of the cell · 2024Article
- The duration of mitosis and daughter cell size are modulated by nutrients in budding yeast.The Journal of cell biology · 2017Article
- Article
- Bud-Localization of CLB2 mRNA Can Constitute a Growth Rate Dependent Daughter Sizer.PLoS computational biology · 2015Article
- PP2ARts1 is a master regulator of pathways that control cell size.The Journal of cell biology · 2014Article
- Complexes of D-type cyclins with CDKs during maize germination.Journal of experimental botany · 2013Article
- Acetyl-CoA induces transcription of the key G1 cyclin CLN3 to promote entry into the cell division cycle in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- PP2A(Cdc55) regulates G1 cyclin stability.Cell cycle (Georgetown, Tex.) · 2013Article
- Plasma membrane growth during the cell cycle: unsolved mysteries and recent progress.Current opinion in cell biology · 2012Review
- A link between mitotic entry and membrane growth suggests a novel model for cell size control.The Journal of cell biology · 2012Article
- Phosphatidylserine is polarized and required for proper Cdc42 localization and for development of cell polarity.Nature cell biology · 2011Article
- The Rts1 regulatory subunit of protein phosphatase 2A is required for control of G1 cyclin transcription and nutrient modulation of cell size.PLoS genetics · 2009Article
- The septins function in G1 pathways that influence the pattern of cell growth in budding yeast.PloS one · 2008Article
- The Dcr2p phosphatase destabilizes Sic1p in Saccharomyces cerevisiae.Biochemical and biophysical research communications · 2007Article
- Use of bimolecular fluorescence complementation to study in vivo interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae.Eukaryotic cell · 2007Article
- Rb, whi it's not just for metazoans anymore.Oncogene · 2006Review
- A role for KEM1 at the START of the cell cycle in Saccharomyces cerevisiae.Current genetics · 2005Article
- Gid8p (Dcr1p) and Dcr2p function in a common pathway to promote START completion in Saccharomyces cerevisiae.Eukaryotic cell · 2004Article
- Hym1p affects cell cycle progression in Saccharomyces cerevisiae.Current genetics · 2004Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Null mutations in three genes encoding cyclin-like proteins (CLN1, CLN2, and CLN3) in Saccharomyces cerevisiae cause cell cycle arrest in G1 (cln arrest). In cln1 cln2 cln3 strains bearing plasmids containing the CLN3 (also called WHI1 or DAF1) coding sequence under the transcriptional control of a galactose-regulated promoter, shift from galactose to glucose medium (shutting off synthesis of CLN3 mRNA) allowed completion of cell cycles in progress but caused arrest in the ensuing unbudded G1 phase. Cell growth was not inhibited in arrested cells. Cell division occurred in glucose medium even if cells were arrested in S phase during the initial 2 h of glucose treatment, suggesting that CLN function may not be required in the cell cycle after S phase. However, when the coding sequence of the hyperactive C-terminal truncation allele CLN3-2 (formerly DAF1-1) was placed under GAL control, cells went through multiple cycles before arresting after a shift from galactose to glucose. These results suggest that the C terminus of the wild-type protein confers functional instability. cln-arrested cells are mating competent. However, cln arrest is distinct from constitutive activation of the mating-factor signalling pathway because cln-arrested cells were dependent on the addition of pheromone both for mating and for induction of an alpha-factor-induced transcript, FUS1, and because MATa/MAT alpha (pheromone-nonresponsive) strains were capable of cln arrest in G1 (although a residual capacity for cell division before arrest was observed in MATa/MAT alpha strains). These results are consistent with a specific CLN requirement for START transit.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.