ArticleProceedings of the National Academy of Sciences of the United States of America2011
Efficient targeted gene disruption in the soma and germ line of the frog Xenopus tropicalis using engineered zinc-finger nucleases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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Who cites it
62 citing papers in PubMed, 132 citations in OpenAlex.
- Application of CRISPR/Cas gene editing for infectious disease control in poultry.Open life sciences · 2025Review
- Cell cycle activation in thyroid hormone-induced apoptosis and stem cell development duringFrontiers in endocrinology · 2023Review
- Steroid-receptor coactivator complexes in thyroid hormone-regulation of Xenopus metamorphosis.Vitamins and hormones · 2023Article
- The development of adult intestinal stem cells: Insights from studies on thyroid hormone-dependent anuran metamorphosis.Vitamins and hormones · 2021Article
- Involvement of epigenetic modifications in thyroid hormone-dependent formation of adult intestinal stem cells during amphibian metamorphosis.General and comparative endocrinology · 2019Article
- The Role of Gene Editing in Neurodegenerative Diseases.Cell transplantation · 2018Review
- Targeted Base Editing via RNA-Guided Cytidine Deaminases in Xenopus laevis Embryos.Molecules and cells · 2017Article
- Noggin is required for first pharyngeal arch differentiation in the frog Xenopus tropicalis.Developmental biology · 2017Article
- Thyroid hormone regulation of adult intestinal stem cells: Implications on intestinal development and homeostasis.Reviews in endocrine & metabolic disorders · 2016Review
- Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos.Asian-Australasian journal of animal sciences · 2016Article
- Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes.Development (Cambridge, England) · 2016Article
- Research proceedings on amphibian model organisms.Dong wu xue yan jiu = Zoological research · 2016Review
- Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation.Marine biotechnology (New York, N.Y.) · 2016Article
- Rapid prototyping of microbial cell factories via genome-scale engineering.Biotechnology advances · 2015Review
- Budgett's frog (Lepidobatrachus laevis): A new amphibian embryo for developmental biology.Developmental biology · 2015Article
- Contractile activity is required for Z-disc sarcomere maturation in vivo.Genesis (New York, N.Y. : 2000) · 2015Article
- Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases.G3 (Bethesda, Md.) · 2015Article
- Targeted mutagenesis of aryl hydrocarbon receptor 2a and 2b genes in Atlantic killifish (Fundulus heteroclitus).Aquatic toxicology (Amsterdam, Netherlands) · 2015Article
- Inbreeding Ratio and Genetic Relationships among Strains of the Western Clawed Frog, Xenopus tropicalis.PloS one · 2015Article
- Finding of a highly efficient ZFN pair for Aqpep gene functioning in murine zygotes.The Journal of reproduction and development · 2015Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
16 authors at 2 institutions in 1 country.
Funding
Abstract
The frog Xenopus, an important research organism in cell and developmental biology, currently lacks tools for targeted mutagenesis. Here, we address this problem by genome editing with zinc-finger nucleases (ZFNs). ZFNs directed against an eGFP transgene in Xenopus tropicalis induced mutations consistent with nonhomologous end joining at the target site, resulting in mosaic loss of the fluorescence phenotype at high frequencies. ZFNs directed against the noggin gene produced tadpoles and adult animals carrying up to 47% disrupted alleles, and founder animals yielded progeny carrying insertions and deletions in the noggin gene with no indication of off-target effects. Furthermore, functional tests demonstrated an allelic series of activity between three germ-line mutant alleles. Because ZFNs can be designed against any locus, our data provide a generally applicable protocol for gene disruption in Xenopus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.