ArticleThe Journal of biological chemistry2011
Genomic selection identifies vertebrate transcription factor Fezf2 binding sites and target genes.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Ehf and Fezf2 regulate late medullary thymic epithelial cell and thymic tuft cell development.Frontiers in immunology · 2023Article
- Article
- The plant-parasitic cyst nematode effector GLAND4 is a DNA-binding protein.Molecular plant pathology · 2018Article
- Control of species-dependent cortico-motoneuronal connections underlying manual dexterity.Science (New York, N.Y.) · 2017Article
- Linc00152 promotes malignant progression of glioma stem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway.Molecular cancer · 2017Article
- Article
- An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and Rapid Restriction of Developmental Potential during Asymmetric Stem Cell Division.Developmental cell · 2017Article
- Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells.The Journal of biological chemistry · 2016Article
- Fezf2 promotes neuronal differentiation through localised activation of Wnt/β-catenin signalling during forebrain development.Development (Cambridge, England) · 2014Article
- Heterogeneously expressed fezf2 patterns gradient Notch activity in balancing the quiescence, proliferation, and differentiation of adult neural stem cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Gene co-regulation by Fezf2 selects neurotransmitter identity and connectivity of corticospinal neurons.Nature neuroscience · 2014Article
- It takes two to tango, a dance between the cells of origin and cancer stem cells in the Drosophila larval brain.Seminars in cell & developmental biology · 2014Review
- Mammalian TBX1 preferentially binds and regulates downstream targets via a tandem T-site repeat.PloS one · 2014Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Identification of transcription factor targets is critical to understanding gene regulatory networks. Here, we uncover transcription factor binding sites and target genes employing systematic evolution of ligands by exponential enrichment (SELEX). Instead of selecting randomly synthesized DNA oligonucleotides as in most SELEX studies, we utilized zebrafish genomic DNA to isolate fragments bound by Fezf2, an evolutionarily conserved gene critical for vertebrate forebrain development. This is, to our knowledge, the first time that SELEX is applied to a vertebrate genome. Computational analysis of bound genomic fragments predicted a core consensus binding site, which identified response elements that mediated Fezf2-dependent transcription both in vitro and in vivo. Fezf2-bound fragments were enriched for conserved sequences. Surprisingly, ∼20% of these fragments overlapped well annotated protein-coding exons. Through loss of function, gain of function, and chromatin immunoprecipitation, we further identified and validated eomesa/tbr2 and lhx2b as biologically relevant target genes of Fezf2. Mutations in eomesa/tbr2 cause microcephaly in humans, whereas lhx2b is a critical regulator of cell fate and axonal targeting in the developing forebrain. These results demonstrate the feasibility of employing genomic SELEX to identify vertebrate transcription factor binding sites and target genes and reveal Fezf2 as a transcription activator and a candidate for evaluation in human microcephaly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.