ReviewBioengineered bugs
Transformation of Saccharomyces cerevisiae and other fungi: methods and possible underlying mechanism.
Review in Bioengineered bugs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed.
- Purine permease 5 contributes to riboflavin distribution in Arabidopsis reproductive organs.Plant & cell physiology · 2026Article
- A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels.Current protocols · 2026Article
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- STIP1/HOP promotes the formation of cytotoxic α-synuclein oligomers.Molecular neurodegeneration advances · 2026Article
- Protein targeting to Starch 2 and the plastidial phosphorylase 1 revealed protein-protein interactions with photosynthesis proteins in yeast two-hybrid screenings.Plant signaling & behavior · 2025Article
- Stress-inducible phosphoprotein 1 (Sti1/Stip1/Hop) sequesters misfolded proteins during stress.The FEBS journal · 2025Article
- Constitutive Promoters Functional in Plant, Fungal, and Bacterial Hosts.ACS synthetic biology · 2025Article
- High-Throughput Indirect Monitoring of TORC1 Activation Using the pTOMAN-G Plasmid in Yeast.Bio-protocol · 2025Article
- Enhancing Yeast Transformation: Achieving up to a Tenfold Increase Through a Single Adjustment in the Lithium Acetate-Polyethylene Glycol Method.Yeast (Chichester, England) · 2025Review
- Article
- Rapid screening system to identify unspecific peroxygenase activity.Clinical hemorheology and microcirculation · 2025Article
- Mining yeast diversity unveils novel targets for improved heterologous laccase production in Saccharomyces cerevisiae.Microbial cell factories · 2025Article
- Atomically accurate de novo design of antibodies with RFdiffusion.bioRxiv : the preprint server for biology · 2025Article
- GmCYP86A37 is a bifunctional cytochrome P450 essential for soybean root aliphatic suberin biosynthesis.Frontiers in plant science · 2025Article
- Glycan masking in immunogen design: computational and experimental methods.Frontiers in immunology · 2025Review
- Overexpression of NAC transcription factors from Eremopyrum triticeum promoted abiotic stress tolerance.Transgenic research · 2024Article
- Development of Green Fluorescent Protein-Tagged Strains ofMicroorganisms · 2024Article
- Phenotyping of a new yeast mapping population reveals differences in the activation of the TORC1 signalling pathway between wild and domesticated yeast strains.Biological research · 2024Article
- Chemical transformation of the multibudding yeast, Aureobasidium pullulans.The Journal of cell biology · 2024Article
- Development of a RobustMicroorganisms · 2024Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transformation (i.e., genetic modification of a cell by the incorporation of exogenous DNA) is indispensable for manipulating fungi. Here, we review the transformation methods for Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans, Pichia pastoris and Aspergillus species and discuss some common modifications to improve transformation efficiency. We also present a model of the mechanism underlying S. cerevisiae transformation, based on recent reports and the mechanism of transfection in mammalian systems. This model predicts that DNA attaches to the cell wall and enters the cell via endocytotic membrane invagination, although how DNA reaches the nucleus is unknown. Polyethylene glycol is indispensable for successful transformation of intact cells and the attachment of DNA and also possibly acts on the membrane to increase the transformation efficiency. Both lithium acetate and heat shock, which enhance the transformation efficiency of intact cells but not that of spheroplasts, probably help DNA to pass through the cell wall.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.