ArticleThe EMBO journal2011
Transcriptome asymmetry within mouse zygotes but not between early embryonic sister blastomeres.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- Variable partition of non-homogeneous ooplasm sets the stage for divergent potency of 2-cell stage blastomeres.Molecular human reproduction · 2026Article
- Animal and vegetal materials of mouse oocytes segregate at first zygotic cleavage: a simple mechanism that makes the two-cell blastomeres differ reciprocally from the start.Molecular human reproduction · 2025Article
- Spatial distribution of maternal factors in pig mature oocytes.Animal biotechnology · 2024Article
- Human embryonic genome activation initiates at the one-cell stage.Cell stem cell · 2022Article
- The impacts of the number of prefreeze and postthaw blastomeres on embryo implantation potential: A systematic analysis.Medicine · 2020Article
- Spatio-temporal expression of ANK2 promotes cytokinesis in oocytes.Scientific reports · 2019Article
- Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification.PloS one · 2019Article
- Article
- Evidence of Oocyte Polarity in Bovine; Implications for Intracytoplasmic Sperm Injection and Somatic Cell Nuclear Transfer.Cell journal · 2017Article
- Totipotency segregates between the sister blastomeres of two-cell stage mouse embryos.Scientific reports · 2017Article
- Polar body transfer restores the developmental potential of oocytes to blastocyst stage in a case of repeated embryo fragmentation.Journal of assisted reproduction and genetics · 2017Article
- Vertebrate Axial Patterning: From Egg to Asymmetry.Advances in experimental medicine and biology · 2017Review
- Article
- Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes.Nature communications · 2016Article
- Article
- Reprogramming the genome to totipotency in mouse embryos.Trends in cell biology · 2015Review
- Origin of cellular asymmetries in the pre-implantation mouse embryo: a hypothesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
- Totipotency: what it is and what it is not.Stem cells and development · 2014Article
- A caffeine fix for human nuclear transfer?Nature biotechnology · 2013Article
- Detection and quantification of maternal-effect gene transcripts in mouse second polar bodies: potential markers of embryo developmental competence.Fertility and sterility · 2013Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Transcriptome regionalization is an essential polarity determinant among metazoans, directing embryonic axis formation during normal development. Although conservation of this principle in mammals is assumed, recent evidence is conflicting and it is not known whether transcriptome asymmetries exist within unfertilized mammalian eggs or between the respective cleavage products of early embryonic divisions. We here address this by comparing transcriptome profiles of paired single cells and sub-cellular structures obtained microsurgically from mouse oocytes and totipotent embryos. Paired microsurgical spindle and remnant samples from unfertilized metaphase II oocytes possessed distinguishable profiles. Fertilization produces a totipotent 1-cell embryo (zygote) and associated spindle-enriched second polar body whose paired profiles also differed, reflecting spindle transcript enrichment. However, there was no programmed transcriptome asymmetry between sister cells within 2- or 3-cell embryos. Accordingly, there is transcriptome asymmetry within mouse oocytes, but not between the sister blastomeres of early embryos. This work places constraints on pre-patterning in mammals and provides documentation correlating potency changes and transcriptome partitioning at the single-cell level.
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Registered trials
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