ArticleAmerican journal of physiology. Endocrinology and metabolism2011
Prolactin-mediated regulation of lipid biosynthesis genes in vivo in the lactating mammary epithelial cell.
Article in American journal of physiology. Endocrinology and metabolism, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Soybean isoflavone promotes milk yield and milk fat yield through the ERα-mediated Akt/mTOR pathway in dairy goats.Journal of animal science · 2024Article
- Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol.Journal of mammary gland biology and neoplasia · 2023Review
- Parental factors that impact the ecology of human mammary development, milk secretion, and milk composition-a report from "Breastmilk Ecology: Genesis of Infant Nutrition (BEGIN)" Working Group 1.The American journal of clinical nutrition · 2023Article
- Protocol for an observational study investigating hormones triggering the onset of sustained lactation: the INSIGHT study.BMJ open · 2022Article
- Polymorphisms of the PRLR Gene and Their Association with Milk Production Traits in Egyptian Buffaloes.Animals : an open access journal from MDPI · 2021Article
- Impact of Maternal Obesity on the Metabolism and Bioavailability of Polyunsaturated Fatty Acids during Pregnancy and Breastfeeding.Nutrients · 2020Review
- Article
- Multi-population GWAS and enrichment analyses reveal novel genomic regions and promising candidate genes underlying bovine milk fatty acid composition.BMC genomics · 2019Article
- Milk lipid regulation at the maternal-offspring interface.Seminars in cell & developmental biology · 2018Review
- Th-POK regulates mammary gland lactation through mTOR-SREBP pathway.PLoS genetics · 2018Article
- Constitutive expression of microRNA-150 in mammary epithelium suppresses secretory activation and impairs de novo lipogenesis.Development (Cambridge, England) · 2016Article
- The glucose transporter Glut1 is selectively essential for CD4 T cell activation and effector function.Cell metabolism · 2014Article
- Biology of glucose transport in the mammary gland.Journal of mammary gland biology and neoplasia · 2014Review
- Cholesterol transport and regulation in the mammary gland.Journal of mammary gland biology and neoplasia · 2014Review
- Regulation of lipid synthesis genes and milk fat production in human mammary epithelial cells during secretory activation.American journal of physiology. Endocrinology and metabolism · 2013Article
- Gene regulation of UDP-galactose synthesis and transport: potential rate-limiting processes in initiation of milk production in humans.American journal of physiology. Endocrinology and metabolism · 2012Article
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Prolactin (PRL) is known to play an essential role in mammary alveolar proliferation in the pregnant mouse, but its role in lactation has been more difficult to define. Genetic manipulations that alter expression of the PRL receptor and its downstream signaling molecules resulted in developmental defects that may directly or indirectly impact secretory activation and lactation. To examine the in vivo role of PRL specifically in lactation, bromocriptine (BrCr) was administered every 8 h to lactating mice on the second day postpartum, resulting in an ~95% decrease in serum PRL levels. Although morphological changes in secretory alveoli were slight, by 8 h of BrCr, pup growth was inhibited significantly. Phosphorylated STAT5 fell to undetectable levels within 4 h. Decreased milk protein gene expression, β-casein, and α-lactalbumin, was observed after 8 h of treatment. To assess mammary-specific effects on lipid synthesis genes, we isolated mammary epithelial cells (MECs) depleted of mammary adipocytes. Expression of genes involved in glucose uptake, glycolysis, pentose phosphate shunt, de novo synthesis of fatty acids, and biosynthesis of triacylglycerides was decreased up to 19-fold in MECs by just 8 h of BrCr treatment. Glands from BrCr-treated mice showed a twofold reduction in intracellular cytoplasmic lipid droplets and a reduction in cytosolic β-casein. These data demonstrate that PRL signaling regulates MEC-specific lipogenic gene expression and that PRL signals coordinate the milk synthesis and mammary epithelial cell survival during lactation in the mouse.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.