ArticleCold Spring Harbor symposia on quantitative biology2010
Insights into interphase large-scale chromatin structure from analysis of engineered chromosome regions.
Article in Cold Spring Harbor symposia on quantitative biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane.PLoS pathogens · 2021Article
- Fiber-Like Organization as a Basic Principle for Euchromatin Higher-Order Structure.Frontiers in cell and developmental biology · 2021Article
- Cytology of DNA Replication Reveals Dynamic Plasticity of Large-Scale Chromatin Fibers.Current biology : CB · 2016Article
- A spotlight on chromatin choreography.The Journal of cell biology · 2015Article
- Unraveling the 3D genome: genomics tools for multiscale exploration.Trends in genetics : TIG · 2015Review
- Single cell visualization of transcription kinetics variance of highly mobile identical genes using 3D nanoimaging.Scientific reports · 2015Article
- Chromatin without the 30-nm fiber: constrained disorder instead of hierarchical folding.Epigenetics · 2014Review
- Large-scale chromatin organization: the good, the surprising, and the still perplexing.Current opinion in cell biology · 2014Review
- Advanced fluorescence microscopy methods for the real-time study of transcription and chromatin dynamics.Transcription · 2014Review
- β-Globin cis-elements determine differential nuclear targeting through epigenetic modifications.The Journal of cell biology · 2013Article
- Review
- Exploring the three-dimensional organization of genomes: interpreting chromatin interaction data.Nature reviews. Genetics · 2013Review
- Chromatin structure outside and inside the nucleus.Biopolymers · 2013Article
- Revisiting higher-order and large-scale chromatin organization.Current opinion in cell biology · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
How chromatin folds into mitotic and interphase chromosomes has remained a difficult question for many years. We have used three generations of engineered chromosome regions as a means of visualizing specific chromosome regions in live cells and cells fixed under conditions that preserve large-scale chromatin structure. Our results confirm the existence of large-scale chromatin domains and fibers formed by the folding of 10-nm and 30-nm chromatin fibers into larger, spatially distinct domains. Transcription at levels within severalfold of the levels measured for endogenous loci occur within these large-scale chromatin structures on a condensed template linearly compacted several hundred fold to 1000-fold relative to B-form DNA. However, transcriptional induction is accompanied by a severalfold decondensation of this large-scale chromatin structure that propagates hundreds of kilobases beyond the induced gene. Examination of engineered chromosome regions in mouse embryonic stem cells (ESCs) and differentiated cells suggests a surprising degree of plasticity in this large-scale chromatin structure, allowing long-range DNA interactions within the context of large-scale chromatin fibers. Recapitulation of gene-specific differences in large-scale chromatin conformation and nuclear positioning using these engineered chromosome regions will facilitate identification of cis and trans determinants of interphase chromosome architecture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.