ArticleThe Journal of biological chemistry2011
Bacterial tubulin distinct loop sequences and primitive assembly properties support its origin from a eukaryotic tubulin ancestor.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 44 citations in OpenAlex.
- The Last Universal Common Ancestor of Ribosome-Encoding Organisms: Portrait of LUCA.Journal of molecular evolution · 2024Review
- Rapid functional activation of horizontally transferred eukaryotic intron-containing genes in the bacterial recipient.Nucleic acids research · 2024Article
- Nanoparticle-assisted tubulin assembly is environment dependent.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A conserved microtubule-binding region in Xanthomonas XopL is indispensable for induced plant cell death reactions.PLoS pathogens · 2023Article
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- Archaea: A Goldmine for Molecular Biologists and Evolutionists.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Multidomain ribosomal protein trees and the planctobacterial origin of neomura (eukaryotes, archaebacteria).Protoplasma · 2020Review
- A microtubule bestiary: structural diversity in tubulin polymers.Molecular biology of the cell · 2017Review
- Bacterial Tubulins A and B Exhibit Polarized Growth, Mixed-Polarity Bundling, and Destabilization by GTP Hydrolysis.Journal of bacteriology · 2017Article
- Four-stranded mini microtubules formed byProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Bacterial kinesin light chain (Bklc) links the Btub cytoskeleton to membranes.Scientific reports · 2017Article
- Six subgroups and extensive recent duplications characterize the evolution of the eukaryotic tubulin protein family.Genome biology and evolution · 2014Article
- The bacterial cytoskeleton: more than twisted filaments.Current opinion in cell biology · 2013Review
- Archaeal origin of tubulin.Biology direct · 2012Article
- Keys to eukaryality: planctomycetes and ancestral evolution of cellular complexity.Frontiers in microbiology · 2012Article
- Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton.PLoS biology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure of the unique bacterial tubulin BtubA/B from Prosthecobacter is very similar to eukaryotic αβ-tubulin but, strikingly, BtubA/B fold without eukaryotic chaperones. Our sequence comparisons indicate that BtubA and BtubB do not really correspond to either α- or β-tubulin but have mosaic sequences with intertwining features from both. Their nucleotide-binding loops are more conserved, and their more divergent sequences correspond to discrete surface zones of tubulin involved in microtubule assembly and binding to eukaryotic cytosolic chaperonin, which is absent from the Prosthecobacter dejongeii draft genome. BtubA/B cooperatively assembles over a wider range of conditions than αβ-tubulin, forming pairs of protofilaments that coalesce into bundles instead of microtubules, and it lacks the ability to differentially interact with divalent cations and bind typical tubulin drugs. Assembled BtubA/B contain close to one bound GTP and GDP. Both BtubA and BtubB subunits hydrolyze GTP, leading to disassembly. The mutant BtubA/B-S144G in the tubulin signature motif GGG(T/S)G(S/T)G has strongly inhibited GTPase, but BtubA-T147G/B does not, suggesting that BtubB is a more active GTPase, like β-tubulin. BtubA/B chimera bearing the β-tubulin loops M, H1-S2, and S9-S10 in BtubB fold, assemble, and have reduced GTPase activity. However, introduction of the α-tubulin loop S9-S10 with its unique eight-residue insertion impaired folding. From the sequence analyses, its primitive assembly features, and the properties of the chimeras, we propose that BtubA/B were acquired shortly after duplication of a spontaneously folding α- and β-tubulin ancestor, possibly by horizontal gene transfer from a primitive eukaryotic cell, followed by divergent evolution.
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