ArticleBMC genomics2011
Expansion and functional diversification of a leucyl aminopeptidase family that encodes the major protein constituents of Drosophila sperm.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Pervasive convergent evolution of sperm conjugation across the Arthropoda tree of life.Nature communications · 2026Review
- Article
- Proteomic diversification of spermatostyles among six species of whirligig beetles.Molecular reproduction and development · 2024Article
- Mitochondrial Differentiation during Spermatogenesis: Lessons fromInternational journal of molecular sciences · 2024Review
- Leucine aminopeptidase1 controls egg deposition and hatchability in male Aedes aegypti mosquitoes.Nature communications · 2024Article
- Gene coexpression network during ontogeny in the yellow fever mosquito, Aedes aegypti.BMC genomics · 2023Article
- Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction.PloS one · 2023Article
- Beneficial commensal bacteria promote Drosophila growth by downregulating the expression of peptidoglycan recognition proteins.iScience · 2022Article
- Review
- Rapid Genomic Evolution Drives the Diversification of Male Reproductive Genes in Dung Beetles.Genome biology and evolution · 2021Article
- Fruit ripening-associated leucylaminopeptidase with cysteinylglycine dipeptidase activity from durian suggests its involvement in glutathione recycling.BMC plant biology · 2021Article
- Recurrent gene co-amplification on Drosophila X and Y chromosomes.PLoS genetics · 2019Article
- Testes Proteases Expression and Hybrid Male Sterility Between Subspecies ofG3 (Bethesda, Md.) · 2019Article
- Proteins, Transcripts, and Genetic Architecture of Seminal Fluid and Sperm in the MosquitoMolecular & cellular proteomics : MCP · 2019Article
- Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm.PLoS genetics · 2019Article
- Positive Selection in the Evolution of Mammalian CRISPs.Journal of molecular evolution · 2018Article
- Contrasting patterns of evolutionary constraint and novelty revealed by comparative sperm proteomic analysis in Lepidoptera.BMC genomics · 2017Article
- Genome Wide Mapping of Peptidases inFrontiers in physiology · 2017Article
- Hybrid male sterility and genome-wide misexpression of male reproductive proteases.Scientific reports · 2015Article
- The genome of the sparganosis tapeworm Spirometra erinaceieuropaei isolated from the biopsy of a migrating brain lesion.Genome biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe evolutionary diversification of gene families through gene creation (and loss) is a dynamic process believed to be critical to the evolution of functional novelty. Previous identification of a closely related family of eight annotated metalloprotease genes of the M17 Merops family in the Drosophila sperm proteome (termed, Sperm-LeucylAminoPeptidases, S-LAPs 1-8) led us to hypothesize that this gene family may have experienced such a diversification during insect evolution.
resultsTo assess putative functional activities of S-LAPs, we (i) demonstrated that all S-LAPs are specifically expressed in the testis, (ii) confirmed their presence in sperm by two-dimensional gel electrophoresis and mass spectrometry, (iii) determined that they represent a major portion of the total protein in sperm and (iv) identified aminopeptidase enzymatic activity in sperm extracts using LAP-specific substrates. Functionally significant divergence at the canonical M17 active site indicates that the largest phylogenetic group of S-LAPs lost catalytic activity and likely acquired novel, as yet undetermined, functions in sperm prior to the expansion of the gene family.
conclusionsComparative genomic and phylogenetic analyses revealed the dramatic expansion of the S-LAP gene family during Drosophila evolution and copy number heterogeneity in the genomes of related insects. This finding, in conjunction with the loss of catalytic activity and potential neofunctionalization amongst some family members, extends empirical support for pervasive "revolving door" turnover in the evolution of reproductive gene family composition and function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.