ArticleBMC genomics2011
Deep sequencing analysis of the developing mouse brain reveals a novel microRNA.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 62 citations in OpenAlex.
- Stage-specific expression patterns and co-targeting relationships among miRNAs in the developing mouse cerebral cortex.Communications biology · 2024Article
- Multiple transcriptome analyses reveal mouse testis developmental dynamics.BMC genomics · 2024Article
- Identification of novel microRNAs in the embryonic mouse brain using deep sequencing.Molecular and cellular biochemistry · 2024Article
- Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission.Science advances · 2022Article
- In the Right Place at the Right Time: miRNAs as Key Regulators in Developing Axons.International journal of molecular sciences · 2020Review
- MicroRNA339 Targeting PDXK Improves Motor Dysfunction and Promotes Neurite Growth in the Remote Cortex Subjected to Spinal Cord Transection.Frontiers in cell and developmental biology · 2020Article
- Effect of Moxibustion on Behavioral Changes and Expression of APP and BACE1 in Hippocampus of SAMP8 Mice.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Temporal dynamics of miRNAs in human DLPFC and its association with miRNA dysregulation in schizophrenia.Translational psychiatry · 2019Article
- Evaluation of methodologies for microRNA biomarker detection by next generation sequencing.RNA biology · 2018Article
- In Silico Prediction and Validation of Gfap as an miR-3099 Target in Mouse Brain.Neuroscience bulletin · 2017Article
- Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing.Malaria journal · 2016Article
- In depth analysis of the Sox4 gene locus that consists of sense and natural antisense transcripts.Data in brief · 2016Article
- PINK1 Deficiency Decreases Expression Levels of mir-326, mir-330, and mir-3099 during Brain Development and Neural Stem Cell Differentiation.Experimental neurobiology · 2016Article
- Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain.Neural plasticity · 2016Article
- An integrative approach to identify hexaploid wheat miRNAome associated with development and tolerance to abiotic stress.BMC genomics · 2015Article
- miR-26a and miR-384-5p are required for LTP maintenance and spine enlargement.Nature communications · 2015Article
- General hallmarks of microRNAs in brain evolution and development.RNA biology · 2015Review
- MiR-3099 is Overexpressed in Differentiating 46c Mouse Embryonic Stem Cells upon Neural Induction.The Malaysian journal of medical sciences : MJMS · 2014Article
- ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain.Nucleic acids research · 2014Article
- Convergent microRNA actions coordinate neocortical development.Cellular and molecular life sciences : CMLS · 2014Review
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) are small non-coding RNAs that can exert multilevel inhibition/repression at a post-transcriptional or protein synthesis level during disease or development. Characterisation of miRNAs in adult mammalian brains by deep sequencing has been reported previously. However, to date, no small RNA profiling of the developing brain has been undertaken using this method. We have performed deep sequencing and small RNA analysis of a developing (E15.5) mouse brain.
resultsWe identified the expression of 294 known miRNAs in the E15.5 developing mouse brain, which were mostly represented by let-7 family and other brain-specific miRNAs such as miR-9 and miR-124. We also discovered 4 putative 22-23 nt miRNAs: mm_br_e15_1181, mm_br_e15_279920, mm_br_e15_96719 and mm_br_e15_294354 each with a 70-76 nt predicted pre-miRNA. We validated the 4 putative miRNAs and further characterised one of them, mm_br_e15_1181, throughout embryogenesis. Mm_br_e15_1181 biogenesis was Dicer1-dependent and was expressed in E3.5 blastocysts and E7 whole embryos. Embryo-wide expression patterns were observed at E9.5 and E11.5 followed by a near complete loss of expression by E13.5, with expression restricted to a specialised layer of cells within the developing and early postnatal brain. Mm_br_e15_1181 was upregulated during neurodifferentiation of P19 teratocarcinoma cells. This novel miRNA has been identified as miR-3099.
conclusionsWe have generated and analysed the first deep sequencing dataset of small RNA sequences of the developing mouse brain. The analysis revealed a novel miRNA, miR-3099, with potential regulatory effects on early embryogenesis, and involvement in neuronal cell differentiation/function in the brain during late embryonic and early neonatal development.
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