ArticleThe Biochemical journal2011
A biochemical analysis of the constraints of tail-anchored protein biogenesis.
Article in The Biochemical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
- Rules of Engagement for Components of Membrane Protein Biogenesis at the Human Endoplasmic Reticulum.International journal of molecular sciences · 2025Review
- Quantitative Mass Spectrometry Characterizes Client Spectra of Components for Targeting of Membrane Proteins to and Their Insertion into the Membrane of the Human ER.International journal of molecular sciences · 2023Review
- The ER Protein Translocation Channel Subunit Sbh1 Controls Virulence of Cryptococcus neoformans.mBio · 2023Article
- The subset of peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved.PloS one · 2023Article
- Signal Peptide Features Determining the Substrate Specificities of Targeting and Translocation Components in Human ER Protein Import.Frontiers in physiology · 2022Review
- SGTA associates with nascent membrane protein precursors.EMBO reports · 2020Article
- A trap mutant reveals the physiological client spectrum of TRC40.Journal of cell science · 2019Article
- Multiple pathways facilitate the biogenesis of mammalian tail-anchored proteins.Journal of cell science · 2017Article
- Mechanistic basis for a molecular triage reaction.Science (New York, N.Y.) · 2017Article
- The Vpu-interacting Protein SGTA Regulates Expression of a Non-glycosylated Tetherin Species.Scientific reports · 2016Article
- mRNA encoding Sec61β, a tail-anchored protein, is localized on the endoplasmic reticulum.Journal of cell science · 2015Article
- Binding of SGTA to Rpn13 selectively modulates protein quality control.Journal of cell science · 2015Article
- Fine-tuning vitamin E-containing telodendrimers for efficient delivery of gambogic acid in colon cancer treatment.Molecular pharmaceutics · 2015Article
- Structural and Functional Insights into Small, Glutamine-Rich, Tetratricopeptide Repeat Protein Alpha.Frontiers in molecular biosciences · 2015Review
- SGTA regulates the cytosolic quality control of hydrophobic substrates.Journal of cell science · 2014Article
- The Get1/2 transmembrane complex is an endoplasmic-reticulum membrane protein insertase.Nature · 2014Article
- Bag6/Bat3/Scythe: a novel chaperone activity with diverse regulatory functions in protein biogenesis and degradation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Review
- Structure of the Sgt2/Get5 complex provides insights into GET-mediated targeting of tail-anchored membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- N-acetylation and phosphorylation of Sec complex subunits in the ER membrane.BMC cell biology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
TA (tail-anchored) proteins utilize distinct biosynthetic pathways, including TRC40 (transmembrane domain recognition complex of 40 kDa)-mediated, chaperone-dependent and/or unassisted routes to the ER (endoplasmic reticulum) membrane. We have addressed the flexibility of cytosolic components participating in these pathways, and explored the thermodynamic constraints of their membrane insertion, by exploiting recombinant forms of Sec61β and Cytb5 (cytochrome b5) bearing covalent modifications within their TA region. In both cases, efficient membrane insertion relied on cytosolic factors capable of accommodating a surprising range of covalent modifications to the TA region. For Sec61β, we found that both SGTA (small glutamine-rich tetratricopeptide repeat-containing protein α) and TRC40 can bind this substrate with a singly PEGylated TA region. However, by introducing two PEG [poly(ethylene glycol)] moieties, TRC40 binding can be prevented, resulting in a block of subsequent membrane integration. Although TRC40 can bind Sec61β polypeptides singly PEGylated at different locations, membrane insertion is more sensitive to the precise location of PEG attachment. Modelling and experimentation indicate that this post-TRC40 effect results from an increased energetic cost of inserting different PEGylated TA regions into the lipid bilayer. We therefore propose that the membrane integration of TA proteins delivered via TRC40 is strongly dependent upon underlying thermodynamics, and speculate that their insertion is via a phospholipid-mediated process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.