ArticleProtein science : a publication of the Protein Society2011
Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents.
Article in Protein science : a publication of the Protein Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- High-throughput cell-free screening of eukaryotic membrane protein expression in lipidic mimetics.Protein science : a publication of the Protein Society · 2022Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Biochemical characterization of a glycosyltransferase Gtf3 from3 Biotech · 2020Article
- Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes.Frontiers in pharmacology · 2018Article
- Production of G protein-coupled receptors in an insect-based cell-free system.Biotechnology and bioengineering · 2017Article
- A Usual G-Protein-Coupled Receptor in Unusual Membranes.Angewandte Chemie (International ed. in English) · 2016Article
- Large-scale production and protein engineering of G protein-coupled receptors for structural studies.Frontiers in pharmacology · 2015Review
- Amphipols for each season.The Journal of membrane biology · 2014Review
- Labeling and functionalizing amphipols for biological applications.The Journal of membrane biology · 2014Review
- Article
- Advances in NMR structures of integral membrane proteins.Current opinion in structural biology · 2013Review
- Calcium-dependent ligand binding and G-protein signaling of family B GPCR parathyroid hormone 1 receptor purified in nanodiscs.ACS chemical biology · 2013Article
- N-terminal fusion tags for effective production of g-protein-coupled receptors in bacterial cell-free systems.Acta naturae · 2012Article
- Facile backbone structure determination of human membrane proteins by NMR spectroscopy.Nature methods · 2012Article
- New amphiphiles for membrane protein structural biology.Methods (San Diego, Calif.) · 2011Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
G-protein coupled receptors (GPCRs) constitute the largest family of intercellular signaling molecules and are estimated to be the target of more than 50% of all modern drugs. As with most integral membrane proteins (IMPs), a major bottleneck in the structural and biochemical analysis of GPCRs is their expression by conventional expression systems. Cell-free (CF) expression provides a relatively new and powerful tool for obtaining preparative amounts of IMPs. However, in the case of GPCRs, insufficient homogeneity of the targeted protein is a problem as the in vitro expression is mainly done with detergents, in which aggregation and solubilization difficulties, as well as problems with proper folding of hydrophilic domains, are common. Here, we report that using CF expression with the help of a fructose-based polymer, NV10 polymer (NVoy), we obtained preparative amounts of homogeneous GPCRs from the three GPCR families. We demonstrate that two GPCR B family members, corticotrophin-releasing factor receptors 1 and 2β are not only solubilized in NVoy but also have functional ligand-binding characteristics with different agonists and antagonists in a detergent-free environment as well. Our findings open new possibilities for functional and structural studies of GPCRs and IMPs in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.