ArticleAnalytical and bioanalytical chemistry2011
Collection, storage, and electrophoretic analysis of nanoliter microdialysis samples collected from awake animals in vivo.
Article in Analytical and bioanalytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- A high-precision view of intercompartmental drug transport via simultaneous, seconds-resolved, in situ measurements in the vein and brain.British journal of pharmacology · 2024Article
- Microdialysis coupled with droplet microfluidics and mass spectrometry for determination of neurotransmittersThe Analyst · 2024Article
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- An Updated Guide to the Identification, Quantitation, and Imaging of the Crustacean Neuropeptidome.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Optimized derivatization of primary amines with the fluorogenic reagent naphthalene-2,3-dicarboxaldehyde toward reproducible quantitative analysis in biological systems.Analytical and bioanalytical chemistry · 2023Article
- High-precision monitoring of and feedback control over drug concentrations in the brains of freely moving rats.Science advances · 2023Article
- Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform.Lab on a chip · 2021Article
- A rotating operant chamber for use with microdialysis.Journal of neuroscience methods · 2019Article
- Label-free time- and space-resolved exometabolite sampling of growing plant roots through nanoporous interfaces.Scientific reports · 2019Article
- Review
- Use and Future Prospects of in Vivo Microdialysis for Epilepsy Studies.ACS chemical neuroscience · 2019Review
- In Vivo Chemical Monitoring at High Spatiotemporal Resolution Using Microfabricated Sampling Probes and Droplet-Based Microfluidics Coupled to Mass Spectrometry.Analytical chemistry · 2018Article
- Measurement of Basal Neurotransmitter Levels Using Convolution-Based Nonfaradaic Current Removal.Analytical chemistry · 2018Article
- Article
- Conceptual and Experimental Tools to Understand Spatial Effects and Transport Phenomena in Nonlinear Biochemical Networks Illustrated with Patchy Switching.Annual review of biochemistry · 2017Review
- Reduced GABAergic transmission in the ventrobasal thalamus contributes to thermal hyperalgesia in chronic inflammatory pain.Scientific reports · 2017Article
- Investigation of signaling molecules and metabolites found in crustacean hemolymph via in vivo microdialysis using a multifaceted mass spectrometric platform.Electrophoresis · 2016Article
- Microfabrication and in Vivo Performance of a Microdialysis Probe with Embedded Membrane.Analytical chemistry · 2016Article
- A review of microdialysis coupled to microchip electrophoresis for monitoring biological events.Journal of chromatography. A · 2015Review
- Nanomaterial-based electrochemical sensing of neurological drugs and neurotransmitters.Mikrochimica acta · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Microdialysis sampling is an important tool for chemical monitoring in living systems. Temporal resolution is an important figure of merit that is determined by sampling frequency, assay sensitivity, and dispersion of chemical zones during transport from sampling device to fraction collector or analytical system. Temporal resolution has recently been improved by segmenting flow into plugs, so that nanoliter fractions are collected at intervals of 0.1-2 s, thus eliminating temporal distortion associated with dispersion in continuous flow. Such systems, however, have yet to be used with behaving subjects. Furthermore, long-term storage of nanoliter samples created by segmented flow has not been reported. In this work, we have addressed these challenges. A microdialysis probe was integrated to a plug generator that could be stably mounted onto behaving animals. Long-term storage of dialysate plugs was achieved by collecting plugs into high-purity perfluoroalkoxy tubes, placing the tube into hexane and then freezing at -80°C. Slow warming with even temperatures prevented plug coalescence during sample thawing. As a demonstration of the system, plugs were collected from the striatum of behaving rats using a 0.5-mm-long microdialysis probe. Resulting plugs were analyzed 1-4 days later by chip-based electrophoresis. To improve throughput of plug analysis over previous work, the speed of electrophoretic separation was increased by using forced air cooling and 1-butyl-2,3-dimethylimidazolium tetrafluoroborate as a separation buffer additive, allowing resolution of six neuroactive amino acids in 30 s. Concentration changes induced by K(+) microinjections were monitored with 10 s temporal resolution. The improvements reported in this work make it possible to apply segmented flow microdialysis to the study of behaving animals and enable experiments where the analytical system cannot be placed close to the animal.
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Registered trials
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