ArticlePloS one2011
In vitro amplification of misfolded prion protein using lysate of cultured cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Amplification of seeding-competent tau aggregates by PMCA in human and experimental tauopathies.Acta neuropathologica communications · 2026Article
- Conventional and State-of-the-Art Detection Methods of Bovine Spongiform Encephalopathy (BSE).International journal of molecular sciences · 2023Review
- Investigating CRISPR/Cas9 gene drive for production of disease-preventing prion gene alleles.PloS one · 2022Article
- Engineering a murine cell line for the stable propagation of hamster prions.The Journal of biological chemistry · 2019Article
- Application of PMCA to screen for prion infection in a human cell line used to produce biological therapeutics.Scientific reports · 2019Article
- In vitro Modeling of Prion Strain Tropism.Viruses · 2019Review
- Asparagine and glutamine ladders promote cross-species prion conversion.The Journal of biological chemistry · 2017Article
- PrPSc formation and clearance as determinants of prion tropism.PLoS pathogens · 2017Article
- PrP Knockout Cells Expressing Transmembrane PrP Resist Prion Infection.Journal of virology · 2017Article
- Glycoform-independent prion conversion by highly efficient, cell-based, protein misfolding cyclic amplification.Scientific reports · 2016Article
- A transfectant RK13 cell line permissive to classical caprine scrapie prion propagation.Prion · 2016Article
- Human prion protein sequence elements impede cross-species chronic wasting disease transmission.The Journal of clinical investigation · 2015Article
- Evaluation of infective property of recombinant prion protein amyloids in cultured cells overexpressing cellular prion protein.Journal of Korean medical science · 2014Article
- Preclinical detection of variant CJD and BSE prions in blood.PLoS pathogens · 2014Article
- A proposed mechanism for the promotion of prion conversion involving a strictly conserved tyrosine residue in the β2-α2 loop of PrPC.The Journal of biological chemistry · 2014Article
- Abrogation of complex glycosylation by swainsonine results in strain- and cell-specific inhibition of prion replication.The Journal of biological chemistry · 2011Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein misfolding cyclic amplification (PMCA) recapitulates the prion protein (PrP) conversion process under cell-free conditions. PMCA was initially established with brain material and then with further simplified constituents such as partially purified and recombinant PrP. However, availability of brain material from some species or brain material from animals with certain mutations or polymorphisms within the PrP gene is often limited. Moreover, preparation of native PrP from mammalian cells and tissues, as well as recombinant PrP from bacterial cells, involves time-consuming purification steps. To establish a convenient and versatile PMCA procedure unrestricted to the availability of substrate sources, we attempted to conduct PMCA with the lysate of cells that express cellular PrP (PrP(C)). PrP(Sc) was efficiently amplified with lysate of rabbit kidney epithelial RK13 cells stably transfected with the mouse or Syrian hamster PrP gene. Furthermore, PMCA was also successful with lysate of other established cell lines of neuronal or non-neuronal origins. Together with the data showing that the abundance of PrP(C) in cell lysate was a critical factor to drive efficient PrP(Sc) amplification, our results demonstrate that cell lysate in which PrP(C) is present abundantly serves as an excellent substrate source for PMCA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.