ArticleProceedings of the National Academy of Sciences of the United States of America2011
Real-time label-free monitoring of adipose-derived stem cell differentiation with electric cell-substrate impedance sensing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
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- Deep Learning-Based Prediction of Individual CellBiosensors · 2025Article
- Label-Free Sorting of Human Mesenchymal Stem Cells Using Insulating Dielectrophoresis.Electrophoresis · 2025Article
- Review
- Deep Learning-Assisted Label-Free Parallel Cell Sorting with Digital Microfluidics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Electrical Impedance Spectroscopy as a Tool to Detect the Epithelial to Mesenchymal Transition in Prostate Cancer Cells.Biosensors · 2024Article
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- Classifying contaminated cell cultures using time series features.Journal of applied statistics · 2024Article
- Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.Stem cell reports · 2023Article
- A simplified workflow with end-point validation of real-time electrical cell-substrate impedance sensing of retinoic acid stimulated neurogenesis in human SH-SY5Y cells in vitro.BMC research notes · 2023Article
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- Combining Electrostimulation with Impedance Sensing to Promote and Track Osteogenesis within a Titanium Implant.Biomedicines · 2023Article
- A Review on Microfluidics-Based Impedance Biosensors.Biosensors · 2023Review
- Novel Approach to Measure Transepithelial Electrical Resistance in Intestinal Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Impedance Imaging of Cells and Tissues: Design and Applications.BME frontiers · 2022Article
- Electric Cell-Substrate Impedance Sensing (ECIS) as a Platform for Evaluating Barrier-Function Susceptibility and Damage from Pulmonary Atelectrauma.Biosensors · 2022Article
- Different Effects of Cigarette Smoke, Heated Tobacco Product and E-Cigarette Vapour on Orbital Fibroblasts in Graves' Orbitopathy; a Study by Real Time Cell Electronic Sensing.Molecules (Basel, Switzerland) · 2022Article
- ECIS Based Electric Fence Method for Measurement of Human Keratinocyte Migration on Different Substrates.Biosensors · 2022Article
- Predicting Cardiovascular Stent Complications Using Self-Reporting Biosensors for Noninvasive Detection of Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Synergistic effect of plasma-activated medium and novel indirubin derivatives on human skin cancer cells by activation of the AhR pathway.Scientific reports · 2022Article
18 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
Real-time monitoring of stem cells (SCs) differentiation will be critical to scale-up SC technologies, while label-free techniques will be desirable to quality-control SCs without precluding their therapeutic potential. We cultured adipose-derived stem cells (ADSCs) on top of multielectrode arrays and measured variations in the complex impedance Z* throughout induction of ADSCs toward osteoblasts and adipocytes. Z* was measured up to 17 d, every 180 s, over a 62.5-64 kHz frequency range with an ECIS Z instrument. We found that osteogenesis and adipogenesis were characterized by distinct Z* time-courses. Significant differences were found (P = 0.007) as soon as 12 h post induction. An increase in the barrier resistance (Rb) up to 1.7 ohm·cm(2) was associated with early osteo-induction, whereas Rb peaked at 0.63 ohm·cm(2) for adipo-induced cells before falling to zero at t = 129 h. Dissimilarities in Z* throughout early induction (<24 h) were essentially attributed to variations in the cell-substrate parameter α. Four days after induction, cell membrane capacitance (Cm) of osteo-induced cells (Cm = 1.72 ± 0.10 μF/cm(2)) was significantly different from that of adipo-induced cells (Cm = 2.25 ± 0.27 μF/cm(2)), indicating that Cm could be used as an early marker of differentiation. Finally, we demonstrated long-term monitoring and measured a shift in the complex plane in the middle frequency range (1 kHz to 8 kHz) between early (t = 100 h) and late induction (t = 380 h). This study demonstrated that the osteoblast and adipocyte lineages have distinct dielectric properties and that such differences can be used to perform real-time label-free quantitative monitoring of adult stem cell differentiation with impedance sensing.
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