ArticleMolecular and cellular biology2011
Genome-wide approaches reveal functional interleukin-4-inducible STAT6 binding to the vascular cell adhesion molecule 1 promoter.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Dupilumab efficacy and safety in patients with moderate to severe asthma: A systematic review and meta-analysis.Frontiers in pharmacology · 2022Pooled it
- Adrenomedullin production by adult cardiac fibroblasts via NF-κB/STAT6 signaling enhances post-infarction lymphangiogenesis and cardiac repair.Scientific reports · 2025Article
- Regulation of eosinophil recruitment and heterogeneity during allergic airway inflammation.Frontiers in allergy · 2025Review
- Association of IL-4 with pachychoroid neovasculopathy.Scientific reports · 2023Article
- When one plus one means more than two: the blockade of both IL-4 and IL-13 inflammatory pathways with dupilumab in a case of severe refractory T2-high asthma.BMJ case reports · 2022Article
- Improving Chromatin-Interaction Prediction Using Single-Cell Open-Chromatin Profiles and Making Insight Into theFrontiers in genetics · 2021Article
- Coordinated demethylation of H3K9 and H3K27 is required for rapid inflammatory responses of endothelial cells.The EMBO journal · 2020Article
- Comprehensive epigenome characterization reveals diverse transcriptional regulation across human vascular endothelial cells.Epigenetics & chromatin · 2019Article
- Dynamically and epigenetically coordinated GATA/ETS/SOX transcription factor expression is indispensable for endothelial cell differentiation.Nucleic acids research · 2017Article
- Transcriptome Profiling in Systems Vascular Medicine.Frontiers in pharmacology · 2017Article
- Comprehensive analysis of promoter-proximal RNA polymerase II pausing across mammalian cell types.Genome biology · 2016Article
- IgE-activated basophils regulate eosinophil tissue entry by modulating endothelial function.The Journal of experimental medicine · 2015Article
- A hypothesis-effect of T cell epitope fusion peptide specific immunotherapy on signal transduction.International journal of clinical and experimental medicine · 2015Article
- Genome-wide approaches reveal functional vascular endothelial growth factor (VEGF)-inducible nuclear factor of activated T cells (NFAT) c1 binding to angiogenesis-related genes in the endothelium.The Journal of biological chemistry · 2014Article
- Cognate interactions: extrafollicular IL-4 drives germinal-center reactions, a new role for an old cytokine.European journal of immunology · 2014Article
- Direct evidence for pitavastatin induced chromatin structure change in the KLF4 gene in endothelial cells.PloS one · 2014Article
- IL-4 attenuates Th1-associated chemokine expression and Th1 trafficking to inflamed tissues and limits pathogen clearance.PloS one · 2013Article
- Cistrome plasticity and mechanisms of cistrome reprogramming.Cell cycle (Georgetown, Tex.) · 2012Review
- Dynamic change of chromatin conformation in response to hypoxia enhances the expression of GLUT3 (SLC2A3) by cooperative interaction of hypoxia-inducible factor 1 and KDM3A.Molecular and cellular biology · 2012Article
- Alternative splicing of CD44 mRNA by ESRP1 enhances lung colonization of metastatic cancer cell.Nature communications · 2012Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Endothelial cell activation and dysfunction underlie many vascular disorders, including atherosclerosis and inflammation. Here, we show that interleukin-4 (IL-4) markedly induced vascular cell adhesion molecule 1 (VCAM-1), both in cultured endothelial cells and in the intact endothelium in mice. Combined treatment with IL-4 and tumor necrosis factor alpha (TNF-α) resulted in further, sustained induction of VCAM-1 expression. IL-4-mediated induction of VCAM-1 and secondary monocyte adhesion was predominantly regulated by the transcription factor STAT6. Genome-wide survey of IL-4-mediated STAT6 binding from sequential chromatin-immunoprecipitation with deep sequencing (chromatin immunoprecipitation sequencing [ChIP-seq]) in endothelial cells revealed regions of transient and sustained transcription factor binding. Through the combination of DNA microarrays and ChIP-seq at the same time points, the majority of IL-4-responsive genes were shown to be STAT6 dependent and associated with direct STAT6 binding to their promoter. IL-4-mediated stable binding of STAT6 led to sustained target gene expression. Moreover, our strategy led to the identification of a novel functionally important STAT6 binding site within 16 kb upstream of the VCAM-1 gene. Taken together, these findings support a critical role for STAT6 in mediating IL-4 signal transduction in endothelial cells. Identification of a novel IL-4-mediated VCAM-1 enhancer may provide a foundation for targeted therapy in vascular disease.
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