ArticleCirculation2011
Nkx2-5 represses Gata1 gene expression and modulates the cellular fate of cardiac progenitors during embryogenesis.
Article in Circulation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Human iPSC-derived self-assembled cardiac organoids for evaluating drug developmental cardiotoxicity.Life medicine · 2025Article
- ETV2 transcriptionally activates Rig1 gene expression and promotes reprogramming of the endothelial lineage.Scientific reports · 2024Article
- Expandable hESC-derived cardiovascular progenitor cells generate functional cardiac lineage cells for microtissue construction.Stem cell research & therapy · 2024Article
- FOXK1 regulates Wnt signalling to promote cardiogenesis.Cardiovascular research · 2023Article
- Genomic Insights into the Origin and Evolution of Molluscan Red-Bloodedness in the Blood Clam Tegillarca granosa.Molecular biology and evolution · 2021Article
- RNAseq shows an all-pervasive day-night rhythm in the transcriptome of the pacemaker of the heart.Scientific reports · 2021Article
- Activating transcription factor 3 coordinates differentiation of cardiac and hematopoietic progenitors by regulating glucose metabolism.Science advances · 2020Article
- Dynamics of cardiomyocyte transcriptome and chromatin landscape demarcates key events of heart development.Genome research · 2019Article
- A new computational method to predict transcriptional activity of a DNA sequence from diverse datasets of massively parallel reporter assays.Nucleic acids research · 2017Article
- Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate.Stem cell reports · 2017Article
- Cardiac Regeneration: Lessons From Development.Circulation research · 2017Review
- Nkx2.5 marks angioblasts that contribute to hemogenic endothelium of the endocardium and dorsal aorta.eLife · 2017Article
- Prediction and validation of protein-protein interactors from genome-wide DNA-binding data using a knowledge-based machine-learning approach.Open biology · 2016Article
- The ETS Factor, ETV2: a Master Regulator for Vascular Endothelial Cell Development.Molecules and cells · 2015Review
- Feedback Mechanisms Regulate Ets Variant 2 (Etv2) Gene Expression and Hematoendothelial Lineages.The Journal of biological chemistry · 2015Article
- Signaling Pathways and Gene Regulatory Networks in Cardiomyocyte Differentiation.Tissue engineering. Part B, Reviews · 2015Review
- A time frame permissive for Protein Kinase D2 activity to direct angiogenesis in mouse embryonic stem cells.Scientific reports · 2015Article
- Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.Stem cells (Dayton, Ohio) · 2015Article
- Article
- Myeloid zinc finger 1 (Mzf1) differentially modulates murine cardiogenesis by interacting with an Nkx2.5 cardiac enhancer.PloS one · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundRecent studies suggest that the hematopoietic and cardiac lineages have close ontogenic origins, and that an early mesodermal cell population has the potential to differentiate into both lineages. Studies also suggest that specification of these lineages is inversely regulated. However, the transcriptional networks that govern the cell fate specification of these progenitors are incompletely defined. METHODS AND
resultsHere, we show that Nkx2-5 regulates the hematopoietic/erythroid fate of the mesoderm precursors early during cardiac morphogenesis. Using transgenic technologies to isolate Nkx2-5 expressing cells, we observed an induction of the erythroid molecular program, including Gata1, in the Nkx2-5-null embryos. We further observed that overexpression of Nkx2-5 with an Nkx2-5-inducible embryonic stem cell system significantly repressed Gata1 gene expression and suppressed the hematopoietic/erythroid potential, but not the endothelial potential, of the embryonic stem cells. This suppression was cell-autonomous, and was partially rescued by overexpressing Gata1. In addition, we demonstrated that Nkx2-5 binds to the Gata1 gene enhancer and represses the transcriptional activity of the Gata1 gene.
conclusionsOur results demonstrate that the hematopoietic/erythroid cell fate is suppressed via Nkx2-5 during mesodermal fate determination, and that the Gata1 gene is one of the targets that are suppressed by Nkx2-5.
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Registered trials
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