ArticleBiochemical and biophysical research communications2011
Osmotic stress alters chromatin condensation and nucleocytoplasmic transport.
Article in Biochemical and biophysical research communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 57 citations in OpenAlex.
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- Large-scale proteomic and phosphoproteomic analysis of erythroid enucleation and maturation.Blood science (Baltimore, Md.) · 2025Article
- Chromatin heterogeneity modulates nuclear condensate dynamics and phase behavior.Nature communications · 2025Article
- Chromatin phase separation and nuclear shape fluctuations are correlated in a polymer model of the nucleus.Nucleus (Austin, Tex.) · 2024Article
- Rethinking nuclear shaping: insights from the nuclear drop model.Soft matter · 2024Review
- The ubiquitin codes in cellular stress responses.Protein & cell · 2024Review
- Visualizing enteric nervous system activity through dye-free dynamic full-field optical coherence tomography.Communications biology · 2023Article
- The Fractal Viewpoint of Tumors and Nanoparticles.Current medicinal chemistry · 2023Review
- The Elephant in the Cell: Nuclear Mechanics and Mechanobiology.Journal of biomechanical engineering · 2022Review
- Who let the DoGs out? - biogenesis of stress-induced readthrough transcripts.Trends in biochemical sciences · 2022Review
- A survey of physical methods for studying nuclear mechanics and mechanobiology.APL bioengineering · 2021Review
- Hyperosmotic stress:Nucleus (Austin, Tex.) · 2020Article
- The Relative Densities of Cytoplasm and Nuclear Compartments Are Robust against Strong Perturbation.Biophysical journal · 2020Article
- Correlating nuclear morphology and external force with combined atomic force microscopy and light sheet imaging separates roles of chromatin and lamin A/C in nuclear mechanics.Molecular biology of the cell · 2020Article
- Nuclear size changes caused by local motion of cell boundaries unfold the nuclear lamina and dilate chromatin and intranuclear bodies.Soft matter · 2019Article
- Article
- Molecular-Level Interactions between Engineered Materials and Cells.International journal of molecular sciences · 2019Review
- Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection.eLife · 2019Article
- Physicochemical Properties of Nucleoli in Live Cells Analyzed by Label-Free Optical Diffraction Tomography.Cells · 2019Article
- Rapid reversible changes in compartments and local chromatin organization revealed by hyperosmotic shock.Genome research · 2019Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Osmotic stress is a potent regulator of biological function in many cell types, but its mechanism of action is only partially understood. In this study, we examined whether changes in extracellular osmolality can alter chromatin condensation and the rate of nucleocytoplasmic transport, as potential mechanisms by which osmotic stress can act. Transport of 10 kDa dextran was measured both within and between the nucleus and the cytoplasm using two different photobleaching methods. A mathematical model was developed to describe fluorescence recovery via nucleocytoplasmic transport. As osmolality increased, the diffusion coefficient of dextran decreased in the cytoplasm, but not the nucleus. Hyper-osmotic stress decreased nuclear size and increased nuclear lacunarity, indicating that while the nucleus was getting smaller, the pores and channels interdigitating the chromatin had expanded. The rate of nucleocytoplasmic transport was increased under hyper-osmotic stress but was insensitive to hypo-osmotic stress, consistent with the nonlinear osmotic properties of the nucleus. The mechanism of this osmotic sensitivity appears to be a change in the size and geometry of the nucleus, resulting in a shorter effective diffusion distance for the nucleus. These results may explain physical mechanisms by which osmotic stress can influence intracellular signaling pathways that rely on nucleocytoplasmic transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.