ArticleBiophysical journal2011
Enhancing the mechanical stability of proteins through a cocktail approach.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Article
- Mechanical forces and ligand binding modulateLife science alliance · 2025Article
- Mechanism for the Unfolding of the TOP7 Protein in Steered Molecular Dynamics Simulations as Revealed by Mutual Information Analysis.Frontiers in molecular biosciences · 2021Article
- A novel strategy for utilizing voice coil servoactuators in tensile tests of low volume protein hydrogels.Macromolecular materials and engineering · 2015Article
- Dual Binding of an Antibody and a Small Molecule Increases the Stability of TERRA G-Quadruplex.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2015Article
- Dual binding of an antibody and a small molecule increases the stability of TERRA G-quadruplex.Angewandte Chemie (International ed. in English) · 2015Article
- Worm-like Ising model for protein mechanical unfolding under the effect of osmolytes.Biophysical journal · 2012Article
- Single-molecule force spectroscopy reveals the individual mechanical unfolding pathways of a surface layer protein.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rationally enhancing the mechanical stability of proteins remains a challenge in the field of single molecule force spectroscopy. Here we demonstrate that it is feasible to use a "cocktail" approach for combining more than one approach to enhance significantly the mechanical stability of proteins in an additive fashion. As a proof of principle, we show that metal chelation and protein-protein interaction can be combined to enhance the unfolding force of a protein to ∼450 pN, which is >3 times of its original value. This is also higher than the mechanical stability of most of proteins studied so far. We also extend such a cocktail concept to combine two different metal chelation sites to enhance protein mechanical stability. This approach opens new avenues to efficiently regulating the mechanical properties of proteins, and should be applicable to a wide range of elastomeric proteins.
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Registered trials
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