ArticleBiophysical journal2011
Spindle pole mechanics studied in mitotic asters: dynamic distribution of spindle forces through compliant linkages.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- The Mechanics of Mitotic Cell Rounding.Frontiers in cell and developmental biology · 2020Review
- The Spindle: Integrating Architecture and Mechanics across Scales.Trends in cell biology · 2018Review
- Cyclin A2 regulates symmetrical mitotic spindle formation and centrosome amplification in human colon cancer cells.American journal of translational research · 2018Article
- Mechanical properties of spindle poles are symmetrically balanced.Biophysics and physicobiology · 2017Article
- Inducible fluorescent speckle microscopy.The Journal of cell biology · 2016Article
- Force on spindle microtubule minus ends moves chromosomes.The Journal of cell biology · 2014Article
- Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension.Journal of cell science · 2013Article
- Maturation of the kinetochore-microtubule interface and the meaning of metaphase.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Review
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
During cell division, chromosomes must faithfully segregate to maintain genome integrity, and this dynamic mechanical process is driven by the macromolecular machinery of the mitotic spindle. However, little is known about spindle mechanics. For example, spindle microtubules are organized by numerous cross-linking proteins yet the mechanical properties of those cross-links remain unexplored. To examine the mechanical properties of microtubule cross-links we applied optical trapping to mitotic asters that form in mammalian mitotic extracts. These asters are foci of microtubules, motors, and microtubule-associated proteins that reflect many of the functional properties of spindle poles and represent centrosome-independent spindle-pole analogs. We observed bidirectional motor-driven microtubule movements, showing that microtubule linkages within asters are remarkably compliant (mean stiffness 0.025 pN/nm) and mediated by only a handful of cross-links. Depleting the motor Eg5 reduced this stiffness, indicating that Eg5 contributes to the mechanical properties of microtubule asters in a manner consistent with its localization to spindle poles in cells. We propose that compliant linkages among microtubules provide a mechanical architecture capable of accommodating microtubule movements and distributing force among microtubules without loss of pole integrity-a mechanical paradigm that may be important throughout the spindle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.