ArticleBiophysical journal2011
Super-resolution imaging of the nucleoid-associated protein HU in Caulobacter crescentus.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Biomolecular condensates as stress sensors and modulators of bacterial signaling.PLoS pathogens · 2024Review
- Structure-Based Analysis of Protein Cluster Size for Super-Resolution Microscopy in the Nervous System.Biomedicines · 2022Article
- DNA-Binding Properties of YbaB, a Putative Nucleoid-Associated Protein FromFrontiers in microbiology · 2021Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- vLUME: 3D virtual reality for single-molecule localization microscopy.Nature methods · 2020Article
- From single bacterial cell imaging towardsEssays in biochemistry · 2019Review
- Absolute Measurements of mRNA Translation in Caulobacter crescentus Reveal Important Fitness Costs of Vitamin BmSystems · 2019Article
- Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging.Molecules (Basel, Switzerland) · 2019Article
- Modified Pearson correlation coefficient for two-color imaging in spherocylindrical cells.BMC bioinformatics · 2018Article
- Minimizing Structural Bias in Single-Molecule Super-Resolution Microscopy.Scientific reports · 2018Article
- Article
- High-resolution 3D models of Caulobacter crescentus chromosome reveal genome structural variability and organization.Nucleic acids research · 2018Article
- Spatial organization and dynamics of RNase E and ribosomes inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like Tail.mBio · 2017Article
- Facilitating trypanosome imaging.Experimental parasitology · 2017Article
- Crowding in Cellular Environments at an Atomistic Level from Computer Simulations.The journal of physical chemistry. B · 2017Article
- Optical and force nanoscopy in microbiology.Nature microbiology · 2016Review
- Cell cycle progression in Caulobacter requires a nucleoid-associated protein with high AT sequence recognition.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Eliminating Fitting from the Modeling of Biological Processes.ACS central science · 2016Article
- Imaging live cells at the nanometer-scale with single-molecule microscopy: obstacles and achievements in experiment optimization for microbiology.Molecules (Basel, Switzerland) · 2014Review
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Authors and funding
5 authors.
Funding
Abstract
Little is known about the structure and function of most nucleoid-associated proteins (NAPs) in bacteria. One reason for this is that the distribution and structure of the proteins is obfuscated by the diffraction limit in standard wide-field and confocal fluorescence imaging. In particular, the distribution of HU, which is the most abundant NAP, has received little attention. In this study, we investigate the distribution of HU in Caulobacter crescentus using a combination of super-resolution fluorescence imaging and spatial point statistics. By simply increasing the laser power, single molecules of the fluorescent protein fusion HU2-eYFP can be made to blink on and off to achieve super-resolution imaging with a single excitation source. Through quantification by Ripley's K-test and comparison with Monte Carlo simulations, we find the protein is slightly clustered within a mostly uniform distribution throughout the swarmer and stalked stages of the cell cycle but more highly clustered in predivisional cells. The methods presented in this letter should be of broad applicability in the future study of prokaryotic NAPs.
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