ArticleBrain structure & function2011
Simultaneous Golgi-Cox and immunofluorescence using confocal microscopy.
Article in Brain structure & function, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Migraine and peripheral pain models show differential alterations in neuronal complexity.Headache · 2022Article
- Expansion-Based Clearing of Golgi-Cox-Stained Tissue for Multi-Scale Imaging.International journal of molecular sciences · 2022Article
- Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens.Scientific reports · 2021Article
- Ocular Dominance Plasticity in Binocular Primary Visual Cortex Does Not Require C1q.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- A Confocal Reflection Super-Resolution Technique to Image Golgi-Cox Stained Neurons.Journal of microscopy · 2019Article
- Estradiol rapidly increases GluA2-mushroom spines and decreases GluA2-filopodia spines in hippocampus CA1.Hippocampus · 2017Article
- Structural and functional plasticity of dendritic spines - root or result of behavior?Genes, brain, and behavior · 2017Review
- Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice.Neurobiology of stress · 2016Article
- Optimising Golgi-Cox staining for use with perfusion-fixed brain tissue validated in the zQ175 mouse model of Huntington's disease.Journal of neuroscience methods · 2016Article
- Hampered long-term depression and thin spine loss in the nucleus accumbens of ethanol-dependent rats.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Therapeutic modalities of squalenoyl nanocomposites in colon cancer: an ongoing search for improved efficacy.ACS nano · 2014Article
- GABAA receptor drugs and neuronal plasticity in reward and aversion: focus on the ventral tegmental area.Frontiers in pharmacology · 2014Article
- The "addicted" spine.Frontiers in neuroanatomy · 2014Review
- Structural plasticity in mesencephalic dopaminergic neurons produced by drugs of abuse: critical role of BDNF and dopamine.Frontiers in pharmacology · 2014Review
- Methods of dendritic spine detection: from Golgi to high-resolution optical imaging.Neuroscience · 2013Review
- Advances in thin tissue Golgi-Cox impregnation: fast, reliable methods for multi-assay analyses in rodent and non-human primate brain.Journal of neuroscience methods · 2013Article
- Regional distribution of 5α-reductase type 2 in the adult rat brain: an immunohistochemical analysis.Psychoneuroendocrinology · 2013Article
- Acute physiological stress promotes clustering of synaptic markers and alters spine morphology in the hippocampus.PloS one · 2013Article
- Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Visualization of neuronal elements is of fundamental importance in modern neuroscience. Golgi-Cox impregnation is a widely employed method that provides detailed information about morphological characteristics of neurons, but none regarding their neurochemical features. Immunocytochemical procedures, on the other hand, can provide a high degree of biochemical specificity but poorer morphological details, in particular if compared to Golgi-Cox impregnation. Hence, the combined use of these two approaches is highly desirable, especially for confocal microscopy that can exploit the advantages of both methods simultaneously. Here we show an innovative procedure of perfusion and fixation of brain tissue, that allows, by applying Golgi-Cox impregnation and immunofluorescence in the same histological section, to obtain high-quality histological material, with a very simple and inexpensive method. This procedure is based on three simple fixation steps: (1) a paraformaldehyde perfusion followed by a standard post-fixation to stabilize the subsequent immunofluorescence reaction; (2) the classical Golgi-Cox impregnation and (3) an immunofluorescence reaction in previously impregnated material. This combination allows simultaneous visualization of (a) the structural details (Golgi-Cox impregnated neurons), (b) the antigens' characterization, (c) the anatomical interactions between discrete neuronal elements and (d) the 3D reconstruction and modeling. The method is easy to perform and can be reproducibly applied by small laboratories and expanded through the use of different antibodies. Overall, the method presented in this study offers an innovative and powerful approach to study the nervous system, especially by using confocal microscopy.
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