ArticleActa crystallographica. Section D, Biological crystallography2011
It's all in the crystals….
Article in Acta crystallographica. Section D, Biological crystallography, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Neutralizing human monoclonal antibodies that target the PcrV component of the type III secretion system ofeLife · 2026Article
- Polymorphic nanobody crystals as long-acting intravitreal therapy for wet age-related macular degeneration.Bioengineering & translational medicine · 2023Article
- Three Molecular Modification Strategies to Improve the Thermostability of Xylanase XynA fromFoods (Basel, Switzerland) · 2023Article
- Article
- BLASTing away preconceptions in crystallization trials.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Designing better diffracting crystals of biotin carboxyl carrier protein from Pyrococcus horikoshii by a mutation based on the crystal-packing propensity of amino acids.Acta crystallographica. Section D, Structural biology · 2017Article
- Design of an expression system to enhance MBP-mediated crystallization.Scientific reports · 2017Article
- Article
- Crystal structures of MBP fusion proteins.Protein science : a publication of the Protein Society · 2016Review
- Increasing the structural coverage of tuberculosis drug targets.Tuberculosis (Edinburgh, Scotland) · 2015Article
- The role of flexibility and molecular shape in the crystallization of proteins by surface mutagenesis.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Enhancing ubiquitin crystallization through surface-entropy reduction.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Article
- Improving the chances of successful protein structure determination with a random forest classifier.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Surface reengineering of RPA70N enables cocrystallization with an inhibitor of the replication protein A interaction motif of ATR interacting protein.Biochemistry · 2013Article
- The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.Structure (London, England : 1993) · 2012Article
- Crystallization chaperone strategies for membrane proteins.Methods (San Diego, Calif.) · 2011Review
- Improvement of the crystallizability and expression of an RNA crystallization chaperone.Journal of biochemistry · 2011Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macromolecular crystallography relies on the availability and quality of single crystals; these are typically obtained through extensive screening, which has a very low intrinsic success rate. Crystallization is not a completely stochastic process and many proteins do not succumb to crystallization because of specific microscopic features of their molecular surfaces. It follows that rational surface engineering through site-directed mutagenesis should allow a systematic and significant improvement in crystallization success rates. Here, one such established strategy, surface-entropy reduction (SER), is discussed, including its successes, limitations and possible future developments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.