ArticleThe Journal of molecular diagnostics : JMD2011
Molecular diagnosis of cystoisosporiasis using extended-range PCR screening.
Article in The Journal of molecular diagnostics : JMD, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- The Brief Case:Journal of clinical microbiology · 2026Review
- Molecular detection of Cystoisospora belli by single-run polymerase chain reaction in stool samples.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2021Article
- Comparison of Two Real-Time PCR Assays Targeting Ribosomal Sequences for the Identification ofPathogens (Basel, Switzerland) · 2021Article
- Epithelial Inclusions in Gallbladder Specimens Mimic Parasite Infection: Histologic and Molecular Examination of Reported Cystoisospora belli Infection in Gallbladders of Immunocompetent Patients.The American journal of surgical pathology · 2018Article
- Article
- Genotypic Identification of Cystoisospora in Immunocompromised Patients Using Tm-Variation Analysis.The Korean journal of parasitology · 2017Article
- Application of stereotactic biopsy for diagnosing intracranial lesions in patients with AIDS in China: Report of 7 cases.Medicine · 2016Article
- Chronic Cystoisospora belli infection in an immunocompetent Myanmar refugee - microscopy is not sensitive enough.BMC infectious diseases · 2016Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Application of a qPCR assay with melting curve analysis for detection and differentiation of protozoan oocysts in human fecal samples from Dominican Republic.The American journal of tropical medicine and hygiene · 2013Article
- Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests.BMC infectious diseases · 2013Review
- Enteric protozoa in the developed world: a public health perspective.Clinical microbiology reviews · 2012Review
- Molecular diagnosis of subcutaneous Pythium insidiosum infection by use of PCR screening and DNA sequencing.Journal of clinical microbiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differential diagnosis of diarrhea in immunocompromised patients encompasses many intestinal parasites including the coccidian Cystoisospora belli. Gastrointestinal infection with C. belli leads to cystoisosporiasis with diarrhea and, depending on host immune status, can cause extraintestinal disease. C. belli is usually diagnosed by examination of stool or intestinal biopsy specimens; however, the organism may be undetected using these test methods. Thus, more sensitive molecular tools for detection of pathogenic parasites are desirable. Herein is described a patient with AIDS who had persistent diarrhea of unknown cause. Microscopic examinations of stool and ileal biopsy specimens were initially unremarkable for any specific pathogen. Screening of DNA extracted from biopsy material using extended-range PCR primers recognizing conserved DNA sequences found in many fungi and parasites revealed infection with C. belli, which was confirmed at repeat histologic analysis. Extended-range PCR screening was used because the differential diagnosis was broad and other tools were not applied, yet this molecular approach led to the appropriate diagnosis and treatment of the condition. Thus, this approach offers a promising test for diagnosis of parasitic diseases that elude diagnosis using conventional methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.