ArticleBritish journal of pharmacology2011
Role of TNF-α in virus-induced airway hyperresponsiveness and neuronal M₂ muscarinic receptor dysfunction.
Article in British journal of pharmacology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Heavy moms, wheezy kids: how maternal obesity harms breathing in offspring.American journal of respiratory cell and molecular biology · 2026Review
- Maternal high-fat diet programs offspring airway hyperinnervation and hyperresponsiveness.JCI insight · 2025Article
- Maternal high-fat diet increases airway sensory innervation and reflex bronchoconstriction in adult offspring.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Bioinformatic analysis and experimental validation of the potential gene in the airway inflammation of steroid-resistant asthma.Scientific reports · 2023Article
- TLR7 is expressed by support cells, but not sensory neurons, in ganglia.Journal of neuroinflammation · 2021Article
- Cholinergic System and Its Therapeutic Importance in Inflammation and Autoimmunity.Frontiers in immunology · 2021Review
- Airway Sensory Nerve Plasticity in Asthma and Chronic Cough.Frontiers in physiology · 2021Review
- Airway cholinergic history modifies mucus secretion properties to subsequent cholinergic challenge in diminished chloride and bicarbonate conditions.Experimental physiology · 2020Article
- The anti-viral facet of anti-rheumatic drugs: Lessons from COVID-19.Journal of autoimmunity · 2020Review
- Clinical characteristics and risk factors associated with COVID-19 disease severity in patients with cancer in Wuhan, China: a multicentre, retrospective, cohort study.The Lancet. Oncology · 2020Article
- Unraveling the connection between eosinophils and obesity.Journal of leukocyte biology · 2020Review
- The mode of action of anticholinergics in asthma.The European respiratory journal · 2018Review
- Eosinophil and airway nerve interactions in asthma.Journal of leukocyte biology · 2018Review
- Newly divided eosinophils limit ozone-induced airway hyperreactivity in nonsensitized guinea pigs.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Protective Role of Eosinophils and TNFa after Ozone Inhalation.Research report (Health Effects Institute) · 2017Article
- Review
- Review
- Anticholinergics/antimuscarinic drugs in asthma.Current allergy and asthma reports · 2014Review
- Interleukin-1β mediates virus-induced m2 muscarinic receptor dysfunction and airway hyperreactivity.American journal of respiratory cell and molecular biology · 2014Article
- Influenza A infection enhances antigen-induced airway inflammation and hyperresponsiveness in young but not aged mice.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
background and purposeInfections with respiratory viruses induce exacerbations of asthma, increase acetylcholine release and potentiate vagally mediated bronchoconstriction by blocking inhibitory M₂ muscarinic receptors on parasympathetic neurons. Here we test whether virus-induced M₂ receptor dysfunction and airway hyperresponsiveness are tumour necrosis factor-alpha (TNF-α) dependent. EXPERIMENTAL APPROACH: Guinea pigs were pretreated with etanercept or phosphate-buffered saline 24 h before intranasal infection with parainfluenza. Four days later, pulmonary inflation pressure, heart rate and blood pressure were measured. M₂ receptor function was assessed by the potentiation by gallamine (an M₂ receptor antagonist) of bronchoconstriction caused by electrical stimulation of the vagus nerves and measured as increased pulmonary inflation pressure. Human airway epithelial cells were infected with influenza and TNF-α concentration in supernatant was measured before supernatant was applied to human neuroblastoma cells. M₂ receptor expression in these neuroblastoma cells was measured by qRT-PCR. KEY
resultsInfluenza-infected animals were hyperresponsive to vagal stimulation but not to intravenous ACh. Gallamine did not potentiate vagally induced bronchoconstriction in virus-infected animals, indicating M₂ receptor dysfunction. Etanercept prevented virus-induced airway hyperresponsiveness and M₂ receptor dysfunction, without changing lung viral titres. Etanercept caused a non-significant decrease in total cells, macrophages and neutrophils in bronchoalveolar lavage. Influenza infection significantly increased TNF-α release from isolated epithelial cells, sufficient to decrease M₂ receptors in neuroblastoma cells. This ability of supernatants from infected epithelial cells to inhibit M₂ receptor expression was blocked by etanercept. CONCLUSIONS AND IMPLICATIONS: TNF-α is a key mediator of virus-induced M₂ muscarinic receptor dysfunction and airway hyperresponsiveness.
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