ArticleAnalytical chemistry2011
Creation of stepwise concentration gradient in picoliter droplets for parallel reactions of matrix metalloproteinase II and IX.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Programmable Control of Nanoliter Droplet Arrays using Membrane Displacement Traps.Advanced materials technologies · 2023Article
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform.Analytical chemistry · 2022Article
- Microfluidic on-demand droplet generation, storage, retrieval, and merging for single-cell pairing.Lab on a chip · 2019Article
- Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Active or Passive On-Demand Droplet Merging in a Microfluidic Valve-Based Trap.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2018Article
- Continuous, One-pot Synthesis and Post-Synthetic Modification of NanoMOFs Using Droplet Nanoreactors.Scientific reports · 2016Article
- Mapping of Enzyme Kinetics on a Microfluidic Device.PloS one · 2016Article
- A barcode-free combinatorial screening platform for matrix metalloproteinase screening.Analytical chemistry · 2015Article
- Electrocoalescence based serial dilution of microfluidic droplets.Biomicrofluidics · 2014Article
- New tools and new biology: recent miniaturized systems for molecular and cellular biology.Molecules and cells · 2013Review
- Controlled dispensing and mixing of pico- to nanoliter volumes using on-demand droplet-based microfluidics.Microfluidics and nanofluidics · 2013Article
- Log-scale dose response of inhibitors on a chip.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We present a new methodology for generating a stepwise concentration gradient in a series of microdroplets by using monolithic micro valves that act as "faucets" in micrometer-scale. A distinct concentration gradient of a substrate was generated for the determination of the kinetic parameters of two different enzymes using only 10 picoliter-scale droplets. With a single experiment on a chip, we obtained K(M) and k(cat) values of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9), and compared the catalytic competence of the two enzymes. The present system and method are highly suitable for applications where the reagents or samples are limited and precious.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.