ArticleThe Journal of comparative neurology2011
Colocalization of hyperpolarization-activated, cyclic nucleotide-gated channel subunits in rat retinal ganglion cells.
Article in The Journal of comparative neurology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Dopaminergic amacrine cells express HCN channels in the developing and adult mouse retina.bioRxiv : the preprint server for biology · 2024Article
- Localization of hyperpolarization-activated cyclic nucleotide-gated channels in the vertebrate retinas across species and their physiological roles.Frontiers in neuroanatomy · 2024Review
- Voltage- and calcium-gated ion channels of neurons in the vertebrate retina.Progress in retinal and eye research · 2019Review
- Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor.Nature communications · 2017Article
- Somatic and neuritic spines on tyrosine hydroxylase-immunopositive cells of rat retina.The Journal of comparative neurology · 2017Article
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- Organ of Corti explants direct tonotopically graded morphology of spiral ganglion neurons in vitro.The Journal of comparative neurology · 2016Article
- Fixation strategies for retinal immunohistochemistry.Progress in retinal and eye research · 2015Review
- Moniliform deformation of retinal ganglion cells by formaldehyde-based fixatives.The Journal of comparative neurology · 2015Article
- I h and HCN channels in murine spiral ganglion neurons: tonotopic variation, local heterogeneity, and kinetic model.Journal of the Association for Research in Otolaryngology : JARO · 2014Article
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- Circadian rhythm of contrast sensitivity is regulated by a dopamine-neuronal PAS-domain protein 2-adenylyl cyclase 1 signaling pathway in retinal ganglion cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Directional summation in non-direction selective retinal ganglion cells.PLoS computational biology · 2013Article
- Hyperpolarization-activated current (In) is reduced in hippocampal neurons from Gabra5-/- mice.PloS one · 2013Article
- Dopamine and full-field illumination activate D1 and D2-D5-type receptors in adult rat retinal ganglion cells.The Journal of comparative neurology · 2012Article
- Thy1 associates with the cation channel subunit HCN4 in adult rat retina.Investigative ophthalmology & visual science · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The current-passing pore of mammalian hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels is formed by subunit isoforms denoted HCN1-4. In various brain areas, antibodies directed against multiple isoforms bind to single neurons, and the current (I(h)) passed during hyperpolarizations differs from that of heterologously expressed homomeric channels. By contrast, retinal rod, cone, and bipolar cells appear to use homomeric HCN channels. Here, we assess the generality of this pattern by examining HCN1 and HCN4 immunoreactivity in rat retinal ganglion cells, measuring I(h) in dissociated cells, and testing whether HCN1 and HCN4 proteins coimmunoprecipitate. Nearly half of the ganglion cells in whole-mounted retinae bound antibodies against both isoforms. Consistent with colocalization and physical association, 8-bromo-cAMP shifted the voltage sensitivity of I(h) less than that of HCN4 channels and more than that of HCN1 channels, and HCN1 coimmunoprecipitated with HCN4 from membrane fraction proteins. Finally, the immunopositive somata ranged in diameter from the smallest to the largest in rat retina, the dendrites of immunopositive cells arborized at various levels of the inner plexiform layer and over fields of different diameters, and I(h) activated with similar kinetics and proportions of fast and slow components in small, medium, and large somata. These results show that different HCN subunits colocalize in single retinal ganglion cells, identify a subunit that can reconcile native I(h) properties with the previously reported presence of HCN4 in these cells, and indicate that I(h) is biophysically similar in morphologically diverse retinal ganglion cells and differs from I(h) in rods, cones, and bipolar cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.